       Document 0669
 DOCN  M9630669
 TI    Type I insulin-like growth factor receptor expression in different
       developmental stages of human thymocytes.
 DT    9603
 AU    Kooijman R; Scholtens LE; Rijkers GT; Zegers BJ; Department of
       Immunology, University Hospital for Children and; Youth Het Wilhelmina
       Kinderziekenhuis, Utrecht, The Netherlands.
 SO    J Endocrinol. 1995 Nov;147(2):203-9. Unique Identifier : AIDSLINE
       MED/96096292
 AB    Insulin-like growth factor-I (IGF-I) has been implicated in playing a
       regulatory role in T cell development and in T cell function. We
       demonstrate the presence of type I IGF receptors on human thymocytes
       using radioligand binding assays and flowcytometric analysis. The
       relative potencies of IGF-I, IGF-II and insulin for competition with
       125I- IGF-I indicate the presence of type I IGF receptors. Scatchard
       analysis revealed that the average number of receptors per thymocyte is
       257 +/- 28 with a Kd of 0.12 +/- 0.01. With multicolour flowcytometry
       using a type I IGF receptor specific monoclonal antibody (alpha IR3), we
       show that CD4-CD8- cells express 3-4 times more receptors per cell as
       compared with CD4+CD8+, CD4+CD8- and CD4-CD8+ cells. IGF-I directly
       stimulated DNA synthesis of thymocytes and potentiated DNA synthesis in
       mitogen-activated thymocytes. These results indicate that IGF-I can
       influence T cell development in humans at the level of the thymus.
 DE    Binding, Competitive  Cell Culture  CD4-Positive
       T-Lymphocytes/METABOLISM  CD8-Positive T-Lymphocytes/METABOLISM
       DNA/BIOSYNTHESIS  Flow Cytometry  Fluorescent Antibody Technique  Human
       Insulin/METABOLISM  Insulin-Like Growth Factor I/METABOLISM/PHARMACOLOGY
       Insulin-Like Growth Factor II/METABOLISM  Radioligand Assay  Receptors,
       Insulin-Like-Growth Factor I/*ANALYSIS/METABOLISM
       T-Lymphocytes/*METABOLISM/PHYSIOLOGY  Thymus Gland/METABOLISM  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

