       Document 0627
 DOCN  M9630627
 TI    Characterization of a CD4-expressing macaque cell line that can detect
       virus after a single replication cycle and can be infected by diverse
       simian immunodeficiency virus isolates.
 DT    9603
 AU    Chackerian B; Haigwood NL; Overbaugh J; Department of Microbiology,
       University of Washington, Seattle; 98195, USA.
 SO    Virology. 1995 Nov 10;213(2):386-94. Unique Identifier : AIDSLINE
       MED/96074514
 AB    Primate lentiviruses such as human immunodeficiency virus (HIV) and
       simian immunodeficiency virus (SIV) are phenotypically diverse, and
       virus isolates vary in cytopathicity, replication rate, and cell
       tropism. While all virus isolates infect primary peripheral blood
       lymphocytes, only a subset of strains infect established CD4-expressing
       T-cell lines. Here, we describe the development and characterization of
       a macaque cell line that can be infected by all of the strains of SIV
       that we have tested, including macrophage- and T-cell-tropic strains,
       primary and cell-line adapted strains, and SIVmac, SIVMne, and SIVsm
       isolates. The cells can be infected by strains of HIV type 2 (HIV-2) to
       varying degrees, but not by either cloned or primary isolates of HIV
       type 1 (HIV-1). This cell line is a derivative of a rhesus macaque
       mammary tumor cell line (CMMT) engineered to express human CD4. For
       these studies, a CMMT-CD4 clone expressing an integrated copy of a
       truncated HIV-1 long terminal repeat fused to the beta-galactosidase
       gene (LTR-beta-gal) was established to allow detection of infectious SIV
       after a single round of replication. Here, we demonstrate the ability of
       the CMMT-CD4-LTR-beta-gal cell line to rapidly and quantitatively detect
       infectious SIV. Using these cells to assay virus, we could readily
       measure neutralizing antibody activity in animals infected with
       different SIV isolates. Neutralizing activity was detected against the
       homologous virus and lower, but detectable, activity was measured
       against heterologous virus. Thus, this system, which is highly sensitive
       and can detect infection by all of the SIV isolates we tested, is a
       rapid method for detecting infectious virus and quantitating
       neutralizing antibody activity.
 DE    beta-Galactosidase/GENETICS  Animal  Antigens, CD4/*ANALYSIS  Clone
       Cells  Genetic Engineering  Human  HIV Long Terminal Repeat
       HIV-1/PHYSIOLOGY  HIV-2/PHYSIOLOGY  Macaca mulatta  Macaca nemestrina
       Mammary Neoplasms/PATHOLOGY  Neutralization Tests  Simian Acquired
       Immunodeficiency Syndrome/IMMUNOLOGY  Support, U.S. Gov't, P.H.S.
       SIV/IMMUNOLOGY/ISOLATION & PURIF/*PHYSIOLOGY  Tumor Cells,
       Cultured/*VIROLOGY  Virion/METABOLISM  *Virus Replication
       Zidovudine/PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

