       Document 0618
 DOCN  M9630618
 TI    Antibody and cytotoxic T-lymphocyte responses to a single
       liposome-associated peptide antigen.
 DT    9603
 AU    White WI; Cassatt DR; Madsen J; Burke SJ; Woods RM; Wassef NM; Alving
       CR; Koenig S; Department of Immunology, MedImmune Incorporated,
       Gaithersburg,; MD 20878, USA.
 SO    Vaccine. 1995 Aug;13(12):1111-22. Unique Identifier : AIDSLINE
       MED/96088067
 AB    The development of peptide-based vaccines that elicit antibody (Ab) and
       cellular immune responses has been hampered by the lack of highly
       immunogenic formulations. In this study, we compared the induction of Ab
       and cytotoxic T-lymphocyte (CTL) responses to a peptide derived from the
       V3 loop of HIV-1 gp120 (P18 and its cysteine-glycine derivative
       (CG-P18)) when incorporated into liposomes with lipid A (LA) or mixed
       with aluminum hydroxide. P18-specific CTL were only observed with
       liposomes with LA. P18-specific Ab responses were found with liposomes
       containing CG-P18 but not P18. Increased surface expression of the
       former, resulted in enhancement of the Ab response without loss of CTL
       induction. Thus, the manner in which a peptide is localized can
       influence the outcome of the response induced by highly immunogenic
       liposome formulations.
 DE    Amino Acid Sequence  Animal  Antibody Formation  Female  HIV Envelope
       Protein gp120/ADMINISTRATION & DOSAGE/*IMMUNOLOGY
       IgG/BLOOD/CLASSIFICATION  Lipid A/ADMINISTRATION & DOSAGE
       Liposomes/ADMINISTRATION & DOSAGE  Mice  Mice, Inbred BALB C  Molecular
       Sequence Data  Peptide Fragments/ADMINISTRATION & DOSAGE/*IMMUNOLOGY
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  T-Lymphocytes,
       Cytotoxic/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

