       Document 0607
 DOCN  M9630607
 TI    Proinflammatory cytokine production and cartilage damage due to
       rheumatoid synovial T helper-1 activation is inhibited by interleukin-4.
 DT    9603
 AU    van Roon JA; van Roy JL; Duits A; Lafeber FP; Bijlsma JW; Department of
       Rheumatology, University Hospital Utrecht, The; Netherlands.
 SO    Ann Rheum Dis. 1995 Oct;54(10):836-40. Unique Identifier : AIDSLINE
       MED/96097726
 AB    OBJECTIVES--To investigate the role of T helper-1 cell (Th1) activation
       in the induction of proinflammatory cytokine production and cartilage
       damage by rheumatoid arthritis (RA) synovial fluid mononuclear cells
       (SFMNC) and the subsequent possible beneficial role of the T helper-2
       cell (Th2) cytokine interleukin-4 (IL-4) in the inhibition of this
       process. METHODS--SFMNC were stimulated with bacterial antigen (hsp60)
       to activate Th1 cells. Th1 and Th2 specific cytokine profiles
       (interferon gamma (IFN gamma) and IL-4) and proinflammatory cytokines
       interleukin-1 (IL-1) and tumour necrosis factor alpha (TNF alpha) in the
       conditioned media were analysed. In addition, the conditioned media were
       tested for their ability to induce cartilage damage. The same parameters
       were measured in the presence of IL-4. RESULTS--Stimulation of SFMNC
       with bacterial antigen resulted in an increase in IFN gamma, IL-1, and
       TNF alpha production which was accompanied by the induction of cartilage
       damage. Th1 activation could be inhibited by IL-4 as shown by a
       reduction of IFN gamma. This was accompanied by a decrease in IL-1 and
       TNF alpha production and inhibition of cartilage damage.
       CONCLUSIONS--Th1 activation is a possible mechanism by which
       inflammation in RA joints is enhanced. The Th2 cytokine IL-4 inhibits
       this Th1 activity and may diminish inflammation and induction of
       cartilage damage in RA joints.
 DE    Adult  Aged  Aged, 80 and over  Antigens, Bacterial/IMMUNOLOGY
       Arthritis, Rheumatoid/*IMMUNOLOGY  Cartilage, Articular/IMMUNOLOGY
       Chaperonin 60/IMMUNOLOGY  Culture Media, Conditioned
       Cytokines/*BIOSYNTHESIS  Female  Human  Interleukin-4/*IMMUNOLOGY
       Lymphocyte Transformation  Male  Middle Age  Support, Non-U.S. Gov't
       Synovial Fluid/*IMMUNOLOGY  Th1 Cells/*IMMUNOLOGY  Tissue Culture
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

