       Document 0598
 DOCN  M9630598
 TI    Acutely infected Langerhans cells are more efficient than T cells in
       disseminating HIV type 1 to activated T cells following a short
       cell-cell contact.
 DT    9603
 AU    Ayehunie S; Groves RW; Bruzzese AM; Ruprecht RM; Kupper TS; Langhoff E;
       Laboratory of Viral Pathogenesis, Dana-Farber Cancer Institute,; Boston,
       Massachusetts 02115, USA.
 SO    AIDS Res Hum Retroviruses. 1995 Aug;11(8):877-84. Unique Identifier :
       AIDSLINE MED/96020088
 AB    Most human immunodeficiency virus type 1 (HIV-1) infections involve
       sexual contact and virus passage across mucosal surfaces. While
       Langerhans cells (LCs) and dendritic cells (DCs) have been implicated in
       mucosal infection, their role is undefined. Here we demonstrate that
       acutely HIV-1-infected LCs and DCs effectively transmit virus to
       uninfected, activated T cells. Cocultivation of these cells results in
       massive virus production that requires a short cell-cell contact; as
       little as 30 min contact time is sufficient for HIV-1-pulsed DCs to
       infect their target T cells. Furthermore, surface-bound virus
       inactivation by trypsin does not significantly decrease the efficiency
       of virus transmission by LC/DCs, suggesting rapid internalization of
       virus. This effective virus transfer by infected LCs and blood-derived
       DCs requires prior activation of T cells. Surprisingly, cocultivation of
       acutely infected T cells with uninfected, activated target T cells
       results only in low virus production, even with T cell-tropic virus. We
       conclude that LCs and DCs are not only important targets of HIV-1
       infection, but may also play a key role in the early dissemination of
       virus to T cells they encounter in skin or lymphoid tissue.
 DE    Cells, Cultured  Coculture  Dendritic Cells/IMMUNOLOGY/*VIROLOGY  Human
       HIV Infections/IMMUNOLOGY/PATHOLOGY/*VIROLOGY  HIV-1/*PHYSIOLOGY
       Langerhans Cells/IMMUNOLOGY/*VIROLOGY  Lymphocyte Transformation
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes/*IMMUNOLOGY/VIROLOGY  Virus Integration  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

