       Document 0592
 DOCN  M9630592
 TI    Induction of potent humoral and cell-mediated immune responses following
       direct injection of DNA encoding the HIV type 1 env and rev gene
       products.
 DT    9603
 AU    Okuda K; Bukawa H; Hamajima K; Kawamoto S; Sekigawa K; Yamada Y; Tanaka
       S; Ishi N; Aoki I; Nakamura M; Department of Bacteriology, Yokohama City
       University School of; Medicine, Japan.
 SO    AIDS Res Hum Retroviruses. 1995 Aug;11(8):933-43. Unique Identifier :
       AIDSLINE MED/96020094
 AB    DNA vaccines have the potential of giving rise to a potent cell-mediated
       immune response by inducing intracellular synthesis and subsequent
       antigenic presentation of encoded antigens. We have tested a DNA vaccine
       specific for human immunodeficiency virus type 1 (HIV-1) by the
       injection of animals with expression plasmids encoding the HIV-1
       envelope protein and the Rev regulatory protein. Injection of both
       plasmids into mice, rabbits, or macaques was found to induce high levels
       of specific antibodies capable of efficiently inhibiting both HIV-1
       infection and envelope-mediated cell fusion. A readily detectable
       delayed-type hypersensitivity (DTH) response was demonstrable in
       injected mice and lymphocytes derived from these proliferated in
       response to an HIV-1 envelope V3 loop-specific peptide. Interestingly,
       the injected mice or macaques also developed a strong cytotoxic T
       lymphocyte (CTL) response against target cells pulsed with the V3
       peptide. Taken together, these data demonstrate that injection of HIV-1
       gene expression plasmids can induce potent humoral and cell-mediated
       immune responses and suggest that DNA vaccines may prove to be
       significantly beneficial as a means of immunizing against HIV-1.
 DE    Animal  Antibody Formation/*IMMUNOLOGY  Base Sequence  DNA,
       Viral/IMMUNOLOGY/*PHARMACOLOGY  Gene Products, rev/GENETICS/*IMMUNOLOGY
       Genes, env/GENETICS/*IMMUNOLOGY  Human  HIV Infections/PREVENTION &
       CONTROL  Immunity, Cellular/*IMMUNOLOGY  Macaca  Mice  Mice, Inbred BALB
       C  Molecular Sequence Data  Plasmids/PHARMACOLOGY  Rabbits  Support,
       Non-U.S. Gov't  Vaccination  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

