       Document 0590
 DOCN  M9630590
 TI    Simian immunodeficiency virus infection of macaque primary placental
       cells.
 DT    9603
 AU    Bui T; Watanabe R; Kennedy B; Unadkat JD; Morton WR; Ho RJ; Department
       of Pharmaceutics, University of Washington, Seattle; 98195, USA.
 SO    AIDS Res Hum Retroviruses. 1995 Aug;11(8):955-61. Unique Identifier :
       AIDSLINE MED/96020096
 AB    We have characterized the ability of a simian immunodeficiency virus,
       SIVmne strain E11S, to infect macaque placental trophoblast and Hofbauer
       cells. These primary placental cells were permissive to SIVmne
       infection, regardless of gestational age. Virus production by the
       infected cells was determined as time-dependent viral core antigen p27
       production, followed by verification of the proviral gag/LTR DNA
       sequences in the infected cells using a polymerase chain reaction assay.
       Of more than six placentas tested, SIVmne infection of placental cells
       at an early gestational age (i.e., days 55 or 78) produced more than
       10-fold the amount of virus core antigen p27 than did placental cells
       infected at a late gestational age (i.e., days 135 or 165). In addition,
       SIVmne infection of trophoblast cells was inhibited by SIVmac
       neutralizing macaque serum but not by normal serum, indicating the
       specificity of virus infection. Furthermore, the amount of SIV core
       antigen p27 produced by the virus-infected trophoblast and Hofbauer
       cells was shown to be dependent on the multiplicity of virus infection.
       Collectively, our results indicate that macaque trophoblast and Hofbauer
       cells can be infected by SIV and that both gestational age and viral
       dose may play a role in the extent of viral infection.
 DE    Animal  Cells, Cultured  Female  Macaca  Placenta/*VIROLOGY  Pregnancy
       Simian Acquired Immunodeficiency Syndrome/*VIROLOGY  Support, U.S.
       Gov't, P.H.S.  SIV/PHYSIOLOGY/*PATHOGENICITY  Viral
       Proteins/BIOSYNTHESIS  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

