       Document 0588
 DOCN  M9630588
 TI    HIV glycoprotein 41 and complement factor H interact with each other and
       share functional as well as antigenic homology.
 DT    9603
 AU    Pinter C; Siccardi AG; Lopalco L; Longhi R; Clivio A; Dipartimento di
       Biologia e Genetica per le Scienze Mediche,; L.I.T.A. Vialba, Universita
       degli Studi di Milano, Italy.
 SO    AIDS Res Hum Retroviruses. 1995 Aug;11(8):971-80. Unique Identifier :
       AIDSLINE MED/96020098
 AB    We have shown that complement factor H (CFH) interacts with HIV-1 at the
       level of the sequence Env 105-119, contained in the C1 domain of gp120.
       CFH interaction with HIV was evident only after dissociation of the Env
       complex induced by exposure to sCD4. We hypothesized that CFH could act
       as a gp41 analog in the interaction with Env 105-119. A panel of
       partially overlapping, synthetic peptides reproducing the extracellular
       portion of gp41 was therefore used to compete the binding of CFH to Env
       105-119. Three sets of peptides that competed this interaction were
       identified. These peptides defined a region of functional homology
       between the gp41 molecule and CFH (Env 580-600), and two regions of
       interaction (Env 620-640 and Env 650-670). In addition to this, a
       monoclonal antibody directed against peptide Env 580-600 and a
       polyclonal mouse antiserum raised against recombinant gp41 were shown to
       recognize CFH in Western blots and ELISA, respectively, also defining a
       region of antigenic homology between gp41 and CFH. These data provide
       evidence for interaction and molecular mimicry between an HIV structural
       protein and a negative regulator of the complement pathway. We show here
       that CFH can interact with both HIV Env proteins, suggesting a possible
       and efficient mechanism of downregulation of the complement cascade at
       the surface of infected cells.
 DE    Amino Acid Sequence  Animal  Complement Factor H/IMMUNOLOGY/*METABOLISM
       Epitopes/IMMUNOLOGY  Human  HIV Envelope Protein
       gp41/CHEMISTRY/IMMUNOLOGY/*METABOLISM  HIV-1/*METABOLISM  Mice
       Molecular Sequence Data  Peptides/CHEMICAL SYNTHESIS/IMMUNOLOGY
       Recombinant Proteins/CHEMISTRY/IMMUNOLOGY/METABOLISM  Sequence Alignment
       Sequence Homology, Amino Acid  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

