       Document 0575
 DOCN  M9630575
 TI    Detection of cytomegalovirus DNA in CD34+ cells from blood and bone
       marrow.
 DT    9603
 AU    von Laer D; Meyer-Koenig U; Serr A; Finke J; Kanz L; Fauser AA;
       Neumann-Haefelin D; Brugger W; Hufert FT; Abteilung Virologie,
       Universitat Freiburg, Germany.
 SO    Blood. 1995 Dec 1;86(11):4086-90. Unique Identifier : AIDSLINE
       MED/96082161
 AB    Infection of hematopoietic progenitor cells with the human
       cytomegalovirus (HCMV) has been proposed as an explanation for the
       cytopenias associated with HCMV-related disease. To test this
       hypothesis, CD34+ cells, which include the hematopoietic progenitors, as
       well as mature leukocyte populations were purified on a
       fluorescence-activated cell sorter and analyzed for HCMV DNA by
       polymerase chain reaction (PCR). A total of 33 samples from 31
       immunosuppressed as well as immunocompetent HCMV-seropositive
       individuals were studied. CD34+ cells were PCR-positive in four of seven
       bone marrow aspirates from allogeneic bone marrow transplant recipients,
       in three of eight aspirates from patients with acquired immunodeficiency
       syndrome, and in the first of two bone marrow samples from an
       immunocompetent patient with primary HCMV disease. CD34+ cells purified
       from peripheral blood for autologous and allogeneic transplantation were
       also analyzed, and 4 of 13 samples were HCMV DNA-positive.
       Interestingly, two of the four HCMV-positive samples were from healthy
       allogeneic donors. Among the mature leukocyte populations, the monocytes
       were most frequently found to be HCMV DNA-positive. No HCMV DNA was
       detected in the total bone marrow leukocytes of 13 healthy seropositive
       bone marrow donors or in the CD34+ cell fraction of three further
       seropositive donors. In conclusion, the data provide strong evidence
       that CD34+ hematopoietic progenitor cells can be infected with HCMV in
       immunosuppressed patients, while this cell population was not identified
       as a major viral reservoir in healthy HCMV-seropositive individuals.
 DE    Acquired Immunodeficiency Syndrome/IMMUNOLOGY/VIROLOGY  Antigens,
       CD34/*METABOLISM  Base Sequence  Bone Marrow Transplantation/IMMUNOLOGY
       Cell Separation  Cytomegalovirus/GENETICS/*ISOLATION & PURIF
       Cytomegalovirus Infections/IMMUNOLOGY/VIROLOGY  DNA Primers/GENETICS
       DNA, Viral/BLOOD/GENETICS/*ISOLATION & PURIF  Flow Cytometry
       Hematopoietic Stem Cells/*IMMUNOLOGY/*VIROLOGY  Human  Immune Tolerance
       Leukocytes/*IMMUNOLOGY/*VIROLOGY  Molecular Sequence Data  Polymerase
       Chain Reaction/STATISTICS & NUMER DATA  Sensitivity and Specificity
       Support, Non-U.S. Gov't  Viremia/IMMUNOLOGY/VIROLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

