       Document 0574
 DOCN  M9630574
 TI    Spontaneous and glucocorticoid-induced apoptosis in human mature T
       lymphocytes.
 DT    9603
 AU    Brunetti M; Martelli N; Colasante A; Piantelli M; Musiani P; Aiello FB;
       Department of Human Pathology, G. D'Annunzio University, Chieti,; Italy.
 SO    Blood. 1995 Dec 1;86(11):4199-205. Unique Identifier : AIDSLINE
       MED/96082175
 AB    Glucocorticoid (GC)-induced apoptosis is a well-recognized physiologic
       regulator of murine T-cell number and function. We have analyzed its
       mechanisms in human mature T cells, which have been thought to be
       insensitive until recently. Peripheral blood T cells showed sensitivity
       to GC-induced apoptosis soon after the proliferative response to a
       mitogenic stimulation, and were also sensitive to spontaneous (ie,
       growth factor deprivation-dependent) apoptosis. CD8+ T cells were more
       sensitive to both forms than CD4+ T cells. Acquisition of sensitivity to
       GC-induced apoptosis was not associated with any change in number or
       affinity of GC receptors. Both spontaneous and GC-induced apoptosis were
       increased by the macromolecular synthesis inhibitors, cycloheximide
       (CHX) and puromycin. A positive correlation between the degree of
       protein synthesis inhibition and the extent of apoptosis was observed.
       Interleukin-2 (IL-2) IL-4, and IL-10 protected (IL-2 > IL-10 > IL-4) T
       cells from both forms of apoptosis in a dose-dependent manner. Our data
       suggest that spontaneous and GC-induced apoptosis regulate the human
       mature T-cell repertoire by acting early after the immune response and
       differentially affecting T-cell subsets.
 DE    Apoptosis/*DRUG EFFECTS  Cell Differentiation
       Cycloheximide/PHARMACOLOGY  CD4-Positive T-Lymphocytes/CYTOLOGY/DRUG
       EFFECTS  CD8-Positive T-Lymphocytes/CYTOLOGY/DRUG EFFECTS
       Dexamethasone/*PHARMACOLOGY  Human  In Vitro  Lymphocyte Transformation
       Protein Synthesis Inhibitors/PHARMACOLOGY  Puromycin/PHARMACOLOGY
       Support, Non-U.S. Gov't  T-Lymphocytes/*CYTOLOGY/*DRUG
       EFFECTS/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

