       Document 0573
 DOCN  M9630573
 TI    Characterization of T cells immortalized by Tax1 of human T-cell
       leukemia virus type 1.
 DT    9603
 AU    Akagi T; Ono H; Shimotohno K; Virology Division, National Cancer Center
       Research Institute,; Tokyo, Japan.
 SO    Blood. 1995 Dec 1;86(11):4243-9. Unique Identifier : AIDSLINE
       MED/96082180
 AB    Peripheral blood T cells were immortalized in vitro by introduction of
       the Tax1 gene of human T-cell leukemia virus type 1 (HTLV-1) with a
       retroviral vector and were characterized for transformation-associated
       markers. Long-term observation showed that these Tax1-immortalized T
       cells eventually exhibited very similar features that were
       characteristic of HTLV-1-immortalized T cells, ie, increased expression
       of egr-1, c-fos, IL-2R alpha, and Lyn and decreased expression of Lck
       and cell-surface CD3 antigen. Among these changes, an increase in the
       expression of Lyn and a decrease in the expression of Lck and
       cell-surface CD3 antigen were observed only in Tax1-immortalized T cells
       after long-term culture. The expression level of Tax1 protein did not
       differ significantly between early and late passage of cells, and the
       cellular clonality was found to be the same by the analysis of the
       retroviral vector integration site and the T-cell receptor beta-chain
       gene rearrangement pattern. These changes in the expression of Lyn, Lck,
       and cell-surface CD3 antigen probably resulted from indirect effects of
       Tax1 that appeared after extended culture.
 DE    Antigens, Differentiation/METABOLISM  Cell Division/DRUG EFFECTS  Cell
       Membrane/IMMUNOLOGY  *Cell Transformation, Viral  Clone Cells  Gene
       Expression  *Genes, pX  Genetic Vectors  Human  HTLV-I/*GENETICS
       Interleukin-2/PHARMACOLOGY  Phenotype  Retroviridae/GENETICS  Support,
       Non-U.S. Gov't  T-Lymphocytes/CYTOLOGY/IMMUNOLOGY/*VIROLOGY  Time
       Factors  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

