       Document 0570
 DOCN  M9630570
 TI    In utero transfer of adult bone marrow cells into recipients with severe
       combined immunodeficiency disorder yields lymphoid progeny with T- and
       B-cell functional capabilities.
 DT    9603
 AU    Blazar BR; Taylor PA; Vallera DA; Department of Pediatrics, University
       of Minnesota Hospital,; Minneapolis 55455, USA.
 SO    Blood. 1995 Dec 1;86(11):4353-66. Unique Identifier : AIDSLINE
       MED/96082194
 AB    To determine if in utero transplantation could restore the immune system
       of mice with a severe combined immunodeficiency (SCID) disorder,
       C57BL/6Sz-scid/scid fetuses were injected on day 14/15 of gestation with
       adult congenic donor bone marrow (BM) cells. Congenic BM engrafted in
       one of eight (13%) recipients. Reconstitution of both lymphoid and
       nonlymphoid lineages was observed. In vitro and in vivo T-cell function
       was documented. Stem cells were shown to have engrafted by secondary
       transfer studies. When fully allogeneic C57BL/6 (H-2b) or B10.BR (H-2k)
       adult. BM cells were given to C.B-17-scid/scid (H-2d) fetal recipients,
       15 of 54 (28%) recipients had evidence of engraftment, with up to 76% of
       peripheral blood (PB) being of in utero donor BM origin on day 131
       postnatally. In all mice with persistent leukocyte engraftment, T- and
       B-lymphoid cells were entirely of donor origin. Donor T cells were
       tolerant to host but not third party alloantigens as measured in vitro.
       In vivo, T-cell function appeared intact. Although most mice had lower
       levels of B-cell engraftment than T-cell engraftment, mice with > or =
       10% B cells were able to produce normal levels of IgM. Despite
       transplantation of fully allogeneic BM cells, stem cell engraftment
       could be demonstrated by secondary transfer of BM cells into lethally
       irradiated recipients that were congenic to the original in utero donor
       BM source. These data indicate that adult BM cells, even those fully
       allogeneic with the fetal recipient, can give rise to progeny with
       multilineage potential, which leads to restoration of T-cell and B-cell
       function.
 DE    Animal  B-Lymphocytes/IMMUNOLOGY  *Bone Marrow Transplantation
       Chimera/IMMUNOLOGY  CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Female  Fetal Diseases/IMMUNOLOGY/*THERAPY
       Graft Survival  Hematopoiesis  Hematopoietic Stem Cells/IMMUNOLOGY  Male
       Mice  Mice, Inbred BALB C  Mice, Inbred C57BL  Mice, SCID  Pregnancy
       Severe Combined Immunodeficiency/IMMUNOLOGY/*THERAPY  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, P.H.S.  Time Factors  Transplantation,
       Homologous  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

