       Document 0543
 DOCN  M9630543
 TI    Detection of human immunodeficiency virus (HIV) type 1 antibodies by new
       automated microparticle enzyme immunoassay for HIV types 1 and 2.
 DT    9603
 AU    Matter L; Germann D; Institute for Medical Microbiology, University of
       Bern,; Switzerland.
 SO    J Clin Microbiol. 1995 Sep;33(9):2338-41. Unique Identifier : AIDSLINE
       MED/96025147
 AB    We compared an automated microparticle double-antigen sandwich enzyme
       immunoassay (EIA) for the IMx test system recently developed by Abbott
       with two established assays (the automated indirect Vidas IgG EIA and
       the double-antigen sandwich EIA from Murex/Wellcome) devised for the
       detection of human immunodeficiency virus type 1 (HIV-1) and HIV-2
       antibodies. A total of 1,078 consecutive serum samples were tested
       prospectively with the three assays. In addition, we used
       retrospectively selected panels of serum samples with discrepant results
       in two different screening tests and with indeterminate or positive
       Western immunoblot (WB) results, as well as five commercially available
       HIV-1 seroconversion panels. The new assay showed excellent
       discriminatory characteristics for the separation of samples from
       HIV-1-positive and HIV-1-negative persons according to Centers for
       Disease Control and Prevention WB criteria. The sensitivities were 98.1,
       92.9, and 96.1% for the new test and the two other assays, respectively,
       and the specificities were 99.7, 97.9, and 98.1%, respectively. With the
       seroconversion panels this new test was positive several days earlier
       than the two other assays; i.e., seroconversion was evident at the peak
       of p24 antigenemia and often several weeks before WB became positive by
       the most stringent criteria.
 DE    Comparative Study  Human  HIV Antibodies/*ANALYSIS/IMMUNOLOGY  HIV
       Infections/*IMMUNOLOGY  HIV-1/*IMMUNOLOGY  HIV-2/*IMMUNOLOGY
       Immunoenzyme Techniques  Predictive Value of Tests  Prospective Studies
       Retrospective Studies  Sensitivity and Specificity  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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