       Document 0542
 DOCN  M9630542
 TI    Detection of Toxoplasma gondii by PCR and tissue culture in
       cerebrospinal fluid and blood of human immunodeficiency
       virus-seropositive patients.
 DT    9603
 AU    Dupon M; Cazenave J; Pellegrin JL; Ragnaud JM; Cheyrou A; Fischer I;
       Leng B; Lacut JY; Service de Medecine Interne, Hopital Pellegrin,
       Bordeaux,; France.
 SO    J Clin Microbiol. 1995 Sep;33(9):2421-6. Unique Identifier : AIDSLINE
       MED/96025163
 AB    To investigate whether both tissue culture and PCR on a sequence from
       the repetitive rDNA could contribute to the diagnosis of toxoplasmosis,
       blood samples and, if they were available, cerebrospinal fluid (CSF) and
       aqueous humor samples from 72 human immunodeficiency virus-seropositive
       patients with suspected toxoplasmosis were prospectively tested. For 10
       patients with fever of unknown origin but without confirmed
       toxoplasmosis, no Toxoplasma gondii was detected. For two patients with
       confirmed toxoplasmic uveitis, only PCR of aqueous humor samples was
       positive. Of 60 patients (48 with CSF samples) with neurological signs,
       25 (from 13 of whom CSF samples were available) had confirmed cerebral
       toxoplasmosis and 10 had a positive PCR of CSF and/or blood samples,
       while for 1 patient culture of the CSF sample was also positive. Unlike
       tissue culture, PCR of rDNA is of value for the detection of cerebral
       toxoplasmosis in human immunodeficiency virus-seropositive patients,
       provided that both CSF and blood samples are available (sensitivity,
       76.9%; specificity, 100%).
 DE    Adolescence  Adult  Aged  Animal  Base Sequence  Female  Human  HIV
       Seropositivity/COMPLICATIONS/*PARASITOLOGY  Male  Middle Age  Molecular
       Sequence Data  Polymerase Chain Reaction  Predictive Value of Tests
       Toxoplasma/*ISOLATION & PURIF
       Toxoplasmosis/COMPLICATIONS/DIAGNOSIS/*PARASITOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

