       Document 0541
 DOCN  M9630541
 TI    Prior enrichment of human immunodeficiency virus DNA with probe DNA
       particles for efficient PCR diagnosis.
 DT    9603
 AU    Tano H; Fan K; Kitajima MA; Kasai K; Tamatsukuri S; Furuichi Y; Hayashi
       T; Kondo M; Imai M; Tsukuba Research Laboratory, Japan Synthetic Rubber
       Co., Ltd.,; Japan.
 SO    J Clin Microbiol. 1995 Sep;33(9):2489-91. Unique Identifier : AIDSLINE
       MED/96025179
 AB    We report here a novel method by which one can recover human
       immunodeficiency virus (HIV) DNA in a small volume (approximately 50
       microliters) of solution from 8 ml of crude cell lysate which contains
       as few as several HIV DNA copies. The method uses the specific binding
       of HIV DNA to HIV probe DNA particles. The HIV DNA thus concentrated on
       the particles can be subjected to the PCR assay. The method also enables
       the examination of 100 individual blood specimens in a combined form.
 DE    Base Sequence  DNA, Viral/*ANALYSIS  Human  HIV/GENETICS/*ISOLATION &
       PURIF  HIV Seropositivity/DIAGNOSIS  Molecular Probe Techniques
       Molecular Sequence Data  Polymerase Chain Reaction/*METHODS  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

