       Document 0536
 DOCN  M9630536
 TI    High-titer packaging cells producing recombinant retroviruses resistant
       to human serum.
 DT    9603
 AU    Cosset FL; Takeuchi Y; Battini JL; Weiss RA; Collins MK; Chester Beatty
       Laboratory, Institute of Cancer Research, London,; United Kingdom.
 SO    J Virol. 1995 Dec;69(12):7430-6. Unique Identifier : AIDSLINE
       GENBANK/X87829
 AB    Novel retroviral protein expression constructs were designed to retain
       minimal retroviral sequences and to express dominant selectable markers
       by reinitiation of translation after expression of the viral genes.
       HT1080 cells were selected as producer cells for their ability to
       release high-titer viruses that are resistant to inactivation by human
       serum. Two HT1080-based packaging cell lines which produce Moloney
       murine leukemia virus cores with envelope glycoproteins of either
       amphotropic murine leukemia virus (FLYA13 line) or cat endogenous virus
       RD114 (FLYRD18 line) are described. Direct comparison with previous
       retroviral packaging systems indicated that 100-fold-higher titers of
       helper-free recombinant viruses were released by the FLYA13 and FLYRD18
       lines.
 DE    Animal  Base Sequence  Blood/*VIROLOGY  Cats  Cell Line  Comparative
       Study  DNA Primers  Genes, env  Genes, pol  Hela Cells  Human  Leukemia
       Viruses, Murine/GENETICS/PHYSIOLOGY  Mice  Molecular Sequence Data
       Moloney Leukemia Virus/GENETICS/PHYSIOLOGY  Polymerase Chain Reaction
       *Recombination, Genetic  Retroviridae/GENETICS/*PHYSIOLOGY  Support,
       Non-U.S. Gov't  Viral Envelope Proteins/*BIOSYNTHESIS/GENETICS  *Virus
       Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

