       Document 0523
 DOCN  M9630523
 TI    Genotypic selection of simian immunodeficiency virus in macaque infants
       infected transplacentally.
 DT    9603
 AU    Amedee AM; Lacour N; Gierman JL; Martin LN; Clements JE; Bohm R Jr;
       Harrison RM; Murphey-Corb M; Tulane Regional Primate Research Center,
       Covington, Louisiana; 70433, USA.
 SO    J Virol. 1995 Dec;69(12):7982-90. Unique Identifier : AIDSLINE
       MED/96079048
 AB    To understand viral and host factors that contribute to transplacental
       transmission of human immunodeficiency virus, we developed an animal
       model using pregnant female macaques infected with simian
       immunodeficiency virus (SIV). Pregnant females were inoculated
       intravenously during midgestation with either a well-characterized
       primary isolate of SIV (SIV/DeltaB670) or a combination of SIV/DeltaB670
       and the macrophage-tropic molecular clone SIV/17E-Fr. The viral genetic
       diversity in five infected female macaques and their in utero-infected
       infants was analyzed. All of the mothers harbored a genetically diverse
       virus population at parturition, whereas a single genotype from the
       maternal quasispecies was identified in the infants at birth. Only one
       of two variants was found in the infants: SIV/17E-Fr (two cases) or a
       genotype contained within the SIV/DeltaB670 quasispecies (three cases).
       The macrophage-tropic properties of both transmitted genotypes were
       suggested by productive replication in primary rhesus macrophage
       cultures in vitro and the clonal presence in central nervous system
       tissue of infected monkeys with encephalitis. These observations provide
       compelling evidence for both genotypic and phenotypic selection in
       transplacental transmission of SIV and suggest a critical role for
       macrophages in fetal infection in utero.
 DE    Acquired Immunodeficiency Syndrome/TRANSMISSION  Amino Acid Sequence
       Animal  Animals, Newborn  Base Sequence  Cloning, Molecular  Comparative
       Study  Consensus Sequence  Disease Transmission, Vertical  DNA Primers
       Female  Genotype  Human  HIV  Lung/VIROLOGY  Lymph Nodes/VIROLOGY
       Macaca fascicularis  Macaca mulatta  Macaca nemestrina  Molecular
       Sequence Data  Phenotype  Polymerase Chain Reaction  Pregnancy
       Pregnancy Complications, Infectious/*VIROLOGY  Proviruses/GENETICS
       Sequence Homology, Amino Acid  Simian Acquired Immunodeficiency
       Syndrome/*TRANSMISSION  Spleen/VIROLOGY  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  SIV/CLASSIFICATION/*GENETICS/ISOLATION &
       PURIF  Thymus Gland/VIROLOGY  Variation (Genetics)  Viral Envelope
       Proteins/CHEMISTRY/*GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

