       Document 0519
 DOCN  M9630519
 TI    CD8+ T-cell epitopes within the surface glycoprotein of a neurotropic
       coronavirus and correlation with pathogenicity.
 DT    9603
 AU    Castro RF; Perlman S; Department of Microbiology, University of Iowa,
       Iowa City 52242,; USA.
 SO    J Virol. 1995 Dec;69(12):8127-31. Unique Identifier : AIDSLINE
       MED/96079072
 AB    CD8+ T cells with cytotoxic activity against the surface glycoprotein
       (S) of mouse hepatitis virus, strain JHM, have been identified in the
       central nervous system (CNS) of both acutely and chronically infected
       C57BL/6 mice. In this report, two specific epitopes recognized by these
       CNS-derived cells were identified, using a panel of peptides chosen
       because they conformed to the allele-specific binding motif for MHC
       class I H-2Kb and H-2Db. The active peptides encompassed residues 510 to
       518 (CSLWNGPHL, H-2Db) and 598 to 605 (RCQIFANI, H-2Kb). Both epitopes
       are located within the region of the S protein previously shown to be
       prone to deletion after passage in animals. These deleted strains are
       generally less neurovirulent than the wild-type virus but still are able
       to cause demyelination. Since C57BL/6 mice become persistently infected
       more commonly than many other strains of mice, these data are consistent
       with a role for CD8+ T-cell escape mutants in the pathogenesis of the
       demyelinating disease. This is the first report of CD8+ T-cell epitope
       localization within the S protein, the protein most strongly implicated
       thus far in pathogenesis in the host.
 DE    Amino Acid Sequence  Animal  Base Sequence
       Brain/*IMMUNOLOGY/PATHOLOGY/*VIROLOGY  Coronavirus
       Infections/*IMMUNOLOGY/PATHOLOGY  Cytotoxicity, Immunologic
       CD8-Positive T-Lymphocytes/*IMMUNOLOGY/VIROLOGY  DNA Primers  DNA,
       Viral/ANALYSIS  Epitopes/*ANALYSIS  Gastroenteritis Virus,
       Murine/*IMMUNOLOGY/ISOLATION & PURIF/  *PATHOGENICITY  Genes, Viral  H-2
       Antigens/CHEMISTRY/*IMMUNOLOGY  Major Histocompatibility Complex  Mice
       Mice, Inbred BALB C  Mice, Inbred C57BL  Molecular Sequence Data
       Mutagenesis  Polymerase Chain Reaction  Sequence Deletion  Support, U.S.
       Gov't, P.H.S.  Viral Structural Proteins/CHEMISTRY/*IMMUNOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

