       Document 0507
 DOCN  M9630507
 TI    Correlation of maternal cytophilic human immunodeficiency virus (HIV)-1
       V3 loop peptide-specific antibodies in infants with vertical HIV
       transmission.
 DT    9603
 AU    Wang XP; Oyaizu N; Pahwa S; Department of Pediatrics, North Shore
       University Hospital-Cornell; University Medical College, Manhasset, New
       York 11030, USA.
 SO    Pediatr Res. 1995 Sep;38(3):384-9. Unique Identifier : AIDSLINE
       MED/96027082
 AB    Approximately 70% of human immunodeficiency virus (HIV)-1-infected
       pregnant women do not transmit HIV to their offsprings. The
       identification of factors involved in maternal-child transmission of HIV
       is important for the design and implementation of therapeutic and
       prevention strategies. Recently we have developed a modified peripheral
       mononuclear cell (PBMC) culture system for in vitro antibody production
       (IVAP) by which we can distinguish serum-derived cytophilic anti-HIV-1
       antibody from de novo synthesized antibody. In this study, we analyzed
       the presence of antibodies directed to HIV-1 gp160, gp120, gp41, and V3
       loop synthetic peptides (C51, C53, and C57 from MN and IIIB strains)
       utilizing the grid-blot method in PBMC cultures of 52 mother-child
       pairs. Among the mothers (39 nontransmitters and 13 transmitters),
       presence of serum-derived cytophilic antibodies or de novo synthesized
       HIV V3 loop peptide-specific antibodies did not correlate with vertical
       transmission. However, PBMC-associated, cytophilic antibodies of
       maternal origin reactive with C51, C53, and C57 from MN and IIIB strains
       were identified in cultures of uninfected infants, but not in infected
       infants. These observations suggest that cytophilic antibodies of
       maternal origin directed to HIV-1 V3 loop peptides which are bound to
       infant cells might play a role in preventing vertical transmission.
 DE    Amino Acid Sequence  *Disease Transmission, Vertical  Enzyme-Linked
       Immunosorbent Assay  Female  Gene Products, env/IMMUNOLOGY  Human  HIV
       Antibodies/*BLOOD  HIV Envelope Protein gp120/*IMMUNOLOGY  HIV
       Infections/*IMMUNOLOGY/*TRANSMISSION  HIV-1/IMMUNOLOGY  *Immunity,
       Maternally-Acquired  Infant  Infant, Newborn  Leukocytes,
       Mononuclear/IMMUNOLOGY  Molecular Sequence Data  Peptide
       Fragments/*IMMUNOLOGY  Pregnancy  Pregnancy Complications, Infectious
       Protein Precursors/IMMUNOLOGY  Sensitivity and Specificity  Support,
       U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

