       Document 0488
 DOCN  M9630488
 TI    Using oligonucleotide probe arrays to access genetic diversity.
 DT    9603
 AU    Lipshutz RJ; Morris D; Chee M; Hubbell E; Kozal MJ; Shah N; Shen N; Yang
       R; Fodor SP; Affymetrix, Santa Clara, CA, USA.
 SO    Biotechniques. 1995 Sep;19(3):442-7. Unique Identifier : AIDSLINE
       MED/96063873
 AB    As the Human Genome Project and related efforts identify and determine
       the DNA sequences of human genes, it is important that highly reliable
       and efficient mechanisms are found to access individual genetic
       variation. It is only through a greater understanding of genetic
       diversity that the true benefit of the Human Genome Project will be
       realized. One approach, hybridization to high-density arrays of
       oligonucleotides, is a fast and effective means of accessing this
       genetic variation. Light-directed chemical synthesis has been used to
       generate miniaturized, high-density arrays of oligonucleotide probes.
       Application-specific oligonucleotide probe array designs have been
       developed for the rapid screening of characterized genes. Dedicated
       instrumentation and software have been developed for array
       hybridization, fluorescence detection and data acquisition and analysis.
       In a specific and challenging application, oligonucleotide probe arrays
       have been used to screen the reverse transcriptase and protease genes of
       the highly polymorphic HIV-1 genome to explore genetic diversity and
       detect mutations conferring resistance to antiviral drugs. Results from
       this application strongly suggest that oligonucleotide probe arrays will
       be a powerful tool for rapid investigations in sequence checking,
       pathogen detection, expression monitoring and DNA molecular recognition.
 DE    Base Sequence  Fluorescent Dyes  Human  HIV-1/GENETICS  Molecular
       Sequence Data  Nucleic Acid Hybridization  *Oligonucleotide Probes
       Polymorphism (Genetics)  Support, U.S. Gov't, Non-P.H.S.  Support, U.S.
       Gov't, P.H.S.  *Variation (Genetics)  JOURNAL ARTICLE  REVIEW  REVIEW,
       TUTORIAL

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

