       Document 0481
 DOCN  M9630481
 TI    CD4-independent in vivo priming of murine CTL by optimal MHC class
       I-restricted peptides derived from intracellular pathogens.
 DT    9603
 AU    Dyall R; Vasovic LV; Molano A; Nikolic-Zugic J; Immunology Program,
       Memorial Sloan-Kettering Cancer Center, New; York 10021, USA.
 SO    Int Immunol. 1995 Aug;7(8):1205-12. Unique Identifier : AIDSLINE
       MED/96022642
 AB    CTL combat intracellular pathogens by killing infected cells. The
       molecular targets of their attack are foreign peptides bound to self MHC
       encoded class I molecules. Immunization of mice with peptides containing
       CTL determinants was shown to elicit CD4-dependent CTL. Here, we have
       achieved in vivo CTL priming with naturally processed 8-10 amino acid
       long class I-restricted peptides emulsified in an adjuvant. A potent,
       reproducible and physiologically relevant response was obtained using
       peptides from an intracellular bacterium and five viruses (including
       HIV) in two murine MHC haplotypes. This method is suitable for multiple
       vaccination, since a 'cocktail' of peptides derived from three pathogens
       elicited effector CTL against each pathogen. Most importantly,
       peptide-induced CD8+CD4- CTL were CD4(+)-independent. These results have
       implications for CTL induction in situations where CD4 T cells are
       depleted or compromised, as is the case in HIV infection.
 DE    Amino Acid Sequence  Animal  Antigens, Bacterial/IMMUNOLOGY  Antigens,
       CD4/*PHYSIOLOGY  Antigens, Viral/IMMUNOLOGY  Cytotoxicity,
       Immunologic/GENETICS  Epitopes  Female  Freund's Adjuvant/PHARMACOLOGY
       H-2 Antigens/*GENETICS/IMMUNOLOGY  Intracellular Fluid/*MICROBIOLOGY
       Lymphocyte Transformation/*GENETICS  Mice  Mice, Inbred C57BL  Mice,
       Inbred DBA  Molecular Sequence Data  Peptides/GENETICS/*IMMUNOLOGY
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  T-Lymphocytes,
       Cytotoxic/CLASSIFICATION/*IMMUNOLOGY/*MICROBIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

