       Document 0476
 DOCN  M9630476
 TI    Differential regulation of IL-13 and IL-4 production by human CD8+ and
       CD4+ Th0, Th1 and Th2 T cell clones and EBV-transformed B cells.
 DT    9603
 AU    de Waal Malefyt R; Abrams JS; Zurawski SM; Lecron JC; Mohan-Peterson S;
       Sanjanwala B; Bennett B; Silver J; de Vries JE; Yssel H; Department of
       Human Immunology, DNAX Research Institute of; Molecular and Cellular
       Biology, Palo Alto, CA 94304-1104, USA.
 SO    Int Immunol. 1995 Sep;7(9):1405-16. Unique Identifier : AIDSLINE
       MED/96091795
 AB    In the present study, the requirements and characteristics for the
       production of IL-13 by human T cells, T cell clones and B cells were
       determined and compared with those of IL-4. IL-13 was produced by human
       CD4+ and CD8+ T lymphocyte subsets isolated from peripheral blood
       mononuclear cells and by CD4+ and CD8+ T cell clones. CD4+ T cell clones
       belonging to Th0, Th1-like and Th2-like subsets produced IL-13 following
       antigen-specific or polyclonal activation. In addition, EBV-transformed
       B cell lines expressed IL-13 mRNA and produced small amounts of IL-13
       protein. Expression of IL-13 mRNA and production of IL-13 protein by
       peripheral blood T cells and T cell clones was induced rapidly and was
       relatively long lasting, whereas IL-4 production by these cells was
       transient. In addition, IL-13 mRNA expression was induced by modes of
       activation that failed to induce IL-4 mRNA expression. IL-13 shares many
       biological activities with IL-4 which is compatible with the notion that
       the IL-13 and IL-4 receptors share a common component required for
       signal transduction. However, IL-13 lacks the T cell-activating
       properties of IL-4. Here we have shown that this is related to the fact
       that T cells fail to bind radiolabeled IL-13 and do not express the
       IL-13-specific receptor component. Taken together, these results
       indicate that the differences in expression and biological activities of
       IL-4 and IL-13 on T cells may have consequences for the relative roles
       of these cytokines in the immune response.
 DE    B-Lymphocytes/*METABOLISM  Base Sequence  Cell Line, Transformed  Clone
       Cells  CD4-Positive T-Lymphocytes/*METABOLISM  CD8-Positive
       T-Lymphocytes/*METABOLISM  Gene Expression Regulation  Herpesvirus 4,
       Human  Human  Interleukin-13/*BIOSYNTHESIS/GENETICS
       Interleukin-4/*BIOSYNTHESIS/GENETICS  Lymphocyte Transformation
       Molecular Sequence Data  Receptors, Interleukin/ANALYSIS  Support,
       Non-U.S. Gov't  Th1 Cells/METABOLISM  Th2 Cells/METABOLISM  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

