       Document 0472
 DOCN  M9630472
 TI    Lysophosphatidylcholine inhibits vesicles fusion induced by the
       NH2-terminal extremity of SIV/HIV fusogenic proteins.
 DT    9603
 AU    Martin I; Ruysschaert JM; Laboratoire de Chimie-Physique des
       Macromolecules aux Interfaces; CP206/2, Universite Libre de Bruxelles,
       Belgium.
 SO    Biochim Biophys Acta. 1995 Nov 22;1240(1):95-100. Unique Identifier :
       AIDSLINE MED/96096712
 AB    Intermediate lipid structures such as inverted micelles and
       interlamellar attachments are thought to play a crucial role in
       different biological processes like exocytosis, intracellular
       trafficking and viral infection. In the present study, we provide
       evidence that lipid mixing of large unilamellar lipid vesicles (LUV)
       mediated by the NH2-terminal sequence of the SIV gp32 and of HIV gp41 is
       inhibited by external addition of lysophosphatidylcholine (lysoPC) to
       LUV containing phosphatidylethanolamine in their lipid bilayer. Leakage
       experiments confirm that lysoPC enhances the stability of the lipids
       organization. The temperature dependence of the two processes as well as
       the complementary shape of PE and lysoPC suggest that the PE-lysoPC
       interaction is involved in the fusion inhibition and stabilization of
       the bilayer.
 DE    Amino Acid Sequence  Cholesterol/METABOLISM  Fluoresceins/METABOLISM
       Gene Products, env/*CHEMISTRY/PHARMACOLOGY  HIV/CHEMISTRY  HIV Envelope
       Protein gp41/*CHEMISTRY/PHARMACOLOGY  Indicators and Reagents/METABOLISM
       Lipid Bilayers/METABOLISM  Liposomes/*METABOLISM
       Lysophosphatidylcholines/*PHARMACOLOGY  Molecular Sequence Data
       Peptides/CHEMISTRY/PHARMACOLOGY  Permeability
       Phosphatidylcholines/METABOLISM  Phosphatidylethanolamines/METABOLISM
       Retroviridae Proteins, Oncogenic/*CHEMISTRY/PHARMACOLOGY  Support,
       Non-U.S. Gov't  SIV/CHEMISTRY  Temperature  Viral Fusion
       Proteins/*CHEMISTRY/*PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

