       Document 0449
 DOCN  M9630449
 TI    Expression of the T antigen on a T-lymphoid cell line, supT1.
 DT    9603
 AU    Nakada H; Inoue M; Tanaka N; Wakamiya N; Yamashina I; Department of
       Biotechnology, Faculty of Engineering, Kyoto,; Japan.
 SO    Glycoconj J. 1995 Jun;12(3):356-9. Unique Identifier : AIDSLINE
       MED/96121958
 AB    We have measured glycosyltransferase activities of SupT1 cells, a
       T-lymphoid cell line shown to react with autoantibodies in the sera of
       many HIV patients. Since considerable
       alpha-N-acetylgalactosaminyl-transferase and beta 1, 3
       galactosyltransferase activities were found in SupT1 cells, at least the
       O-glycan core 1 structure can probably be synthesized. FACS analysis
       using an anti-T monoclonal antibody showed expression of the T antigen
       (Gal beta 1-3 GalNAc). Glycoproteins with the T antigen were isolated by
       immunoprecipitation with the anti-T antibody from a SupT1 cell lysate
       labelled metabolically with 3H-glucosamine and then analysed by
       SDS-PAGE. It was revealed that the precipitate contained a glycoprotein
       with a molecular weight corresponding to that of leukosialin. O-glycans
       were prepared from the immunoprecipitate by alkaline-borohydride
       treatment and then fractionated on Bio-Gel P-2, GalNAcOH and
       Gal-GalNAcOH being identified inter alia. These results suggest that an
       anti-T antibody may be included in the autoantibodies found in HIV-1
       infected individuals.
 DE    Antigens, CD/*BLOOD  Antigens, Viral, Tumor/*BLOOD  Autoantibodies/BLOOD
       Carbohydrate Sequence  Cell Line  Human  HIV-1/*IMMUNOLOGY  Molecular
       Sequence Data  Polysaccharides/BLOOD  Sialoglycoproteins/*IMMUNOLOGY
       Support, Non-U.S. Gov't  T-Lymphocytes/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

