       Document 0436
 DOCN  M9630436
 TI    Evidence for anti-Plasmodium falciparum antibodies that cross-react with
       human T-lymphotropic virus type I proteins in a population in Irian
       Jaya, Indonesia.
 DT    9603
 AU    Porter KR; Liang L; Long GW; Bangs MJ; Anthony R; Andersen EM; Hayes CG;
       Infectious Diseases Threat Assessment Program, Naval Medical; Research
       Institute, Bethesda, Maryland 20852, USA.
 SO    Clin Diagn Lab Immunol. 1994 Jan;1(1):11-5. Unique Identifier : AIDSLINE
       MED/96050771
 AB    This study was performed to demonstrate the presence of anti-Plasmodium
       falciparum antibodies in a population living in Irian Jaya, Indonesia
       that cross-react with human T-lymphotropic virus type I (HTLV-I)
       proteins. Serum samples from 63 volunteers living in Oksibil, a secluded
       highland valley in Irian Jaya, were tested for anti-P. falciparum
       antibodies by an immunofluorescence assay and for anti-HTLV-I antibodies
       by an enzyme immunoassay (EIA). All samples were positive for anti-P.
       falciparum antibodies at titers of > or = 1:256. Twenty-four samples
       were reactive by EIA for HTLV-I, and of these, 23 were tested by western
       blotting (immunoblotting). Five of the 23 samples were classified as
       western blot positive and 18 were classified as western blot
       indeterminate. In competitive blocking assays with malaria proteins,
       western blot immunoreactivity to all HTLV-I Gag proteins was either
       reduced or eliminated. Significant reductions in the HTLV-I EIA optical
       density values of the Oksibil sera occurred when the sera were
       competitively blocked with the malaria antigens. The optical density
       values of HTLV-I-positive control sera showed no significant change.
       Competitive blocking with HTLV-I antigens produced reductions in the
       optical density values of both the Oksibil sera and the HTLV-I-positive
       control sera. These data suggest that in this population, anti-P.
       falciparum antibodies are cross-reactive with HTLV-I proteins in the
       western blot and EIA tests.
 DE    Animal  Antibodies, Protozoan/*CHEMISTRY  Binding, Competitive  Cross
       Reactions  Human  HTLV-I Antibodies/CHEMISTRY  HTLV-I
       Antigens/*IMMUNOLOGY  Indonesia/EPIDEMIOLOGY  Malaria,
       Falciparum/EPIDEMIOLOGY/IMMUNOLOGY/VIROLOGY  Plasmodium
       falciparum/*IMMUNOLOGY  Support, U.S. Gov't, Non-P.H.S.  Viral
       Proteins/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

