       Document 0429
 DOCN  M9630429
 TI    Effect of specimen storage on absolute CD4 counts.
 DT    9603
 AU    Kabengera S; Hom D; Jackson B; Uganda Cancer Institute, Kampala, Uganda.
 SO    Clin Diagn Lab Immunol. 1994 Mar;1(2):232-4. Unique Identifier :
       AIDSLINE MED/96050812
 AB    We have evaluated the effect of specimen storage on absolute CD4 counts
       by a commercially available manual assay. This assay utilizes latex
       particles coated with CD4 monoclonal antibodies that are mixed with
       lymphocytes in whole blood. Thirty blood samples were analyzed on days
       1, 2, 4, and 7 postcollection. Linear regression analysis and Pearson
       correlation coefficients were used to determine the relationship between
       the absolute CD4 count and the storage time after sample collection.
       There was a significant decrease in absolute CD4 counts from baseline
       over time, dropping 3.6% at day 2, 10.1% at day 4, and 18.8% at day 7.
       However, the standard error of the B coefficient was constant [SE (B) =
       0.031] up to day 4, indicating that reliable estimates of the baseline
       CD4 counts could be made from the CD4 counts determined up to day 4 from
       the time of sample collection. In addition to being sample, rapid, and
       inexpensive, the manual assay is capable of giving a reliable absolute
       CD4 count after specimen storage of up to 4 days. The application of
       this assay in the limited facilities of developing countries'
       laboratories is attractive.
 DE    *Blood Specimen Collection  *CD4 Lymphocyte Count/METHODS  Human
       *Preservation, Biological  Reference Values  Reproducibility of Results
       Specimen Handling/ADVERSE EFFECTS  Support, U.S. Gov't, P.H.S.
       Temperature  Time Factors  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

