       Document 0428
 DOCN  M9630428
 TI    Spontaneous lymphocyte proliferation in human T-cell lymphotropic virus
       type I (HTLV-I) and HTLV-II infection: T-cell subset responses and their
       relationships to the presence of provirus and viral antigen production.
 DT    9603
 AU    Prince HE; York J; Golding J; Owen SM; Lal RB; Cellular Immunology
       Laboratory, American Red Cross Blood and; Tissue Services, Los Angeles,
       California 90006, USA.
 SO    Clin Diagn Lab Immunol. 1994 May;1(3):273-82. Unique Identifier :
       AIDSLINE MED/96050823
 AB    Spontaneous lymphocyte proliferation (SLP) during in vitro culture of
       mononuclear cells (MCs) characterizes over half of asymptomatic
       individuals infected with human T-cell lymphotropic virus type I
       (HTLV-I) or HTLV-II. Both CD4 and CD8 T-cell subsets within MC cultures
       are activated during SLP, as judged by high-density CD25 (CD25bright)
       expression; it is unclear, however, whether both cell subsets can
       directly undergo SLP. In the present investigation, the SLP capacities
       of purified CD8 and CD4 cells were examined in subjects infected with
       HTLV-I (n = 19) or HTLV-II (n = 54) in relation to the SLP status of MCs
       from each subject. No increase in SLP was observed for CD8 or CD4 cells
       from SLP-negative (SLP-) HTLV-infected subjects, whereas robust SLP
       characterized CD8 cells from all SLP-positive (SLP+) individuals,
       regardless of HTLV type. In contrast, SLP+ CD4 cells characterized only
       23% (7 of 31) of HTLV-II+ SLP+ individuals, whereas SLP+ CD4 cells
       characterized 100% of HTLV-I+ SLP+ individuals. In cocultures of
       HTLV-II+ SLP+ CD8 cells and autologous SLP- CD4 cells, sizable
       proportions of both CD8 cells and CD4 cells coexpressed CD25bright,
       suggesting that SLP- CD4 cells were activated in the presence of SLP+
       CD8 cells. PCR analysis for tax sequences detected provirus in most CD4-
       and CD8-cell preparations from HTLV-seropositive individuals, regardless
       of type and the SLP status of cell subsets. To determine whether SLP was
       associated with activation of viral genes, levels of HTLV-I and HTLV-II
       core antigen (Ag) in supernatants were measured. Viral Ag production and
       SLP responses were significantly correlated for both CD4 and CD8 cells
       in both HTLV-I and HTLV-II infections. However, inhibition of CD8- or
       CD4-cell SLP by cyclosporin A or anti-Tac (anti-CD25) did not reduce Ag
       production, indicating that Ag production is not coupled to SLP. These
       findings show that CD4 cells from SLP+ HTLV-I+ and SLP+ HTLV-II+
       individuals differ in SLP capacity, that the absence of SLP does not
       indicate a lack of infection, and that production of viral Ag is
       associated with, but not dependent on, SLP.
 DE    Base Sequence  CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Genome, Viral  Human  HTLV-I
       Antigens/*BIOSYNTHESIS  HTLV-I Infections/*IMMUNOLOGY  HTLV-II
       Antigens/*BIOSYNTHESIS  HTLV-II Infections/*IMMUNOLOGY  Leukocytes,
       Mononuclear/IMMUNOLOGY  *Lymphocyte Transformation  Molecular Sequence
       Data  Monocytes/IMMUNOLOGY  Proviruses/*IMMUNOLOGY  Support, Non-U.S.
       Gov't  T-Lymphocyte Subsets/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

