       Document 0427
 DOCN  M9630427
 TI    Use of fixed autologous stimulator cells to correctly present human
       immunodeficiency virus type 1 viral peptides to nonhuman primate
       lymphocytes in proliferation and cytotoxic T-lymphocyte assays.
 DT    9603
 AU    Munroe KJ; Anderson CA; Wu JY; Wyand MS; Newman GW; Newman MJ; Cambridge
       Biotech Corporation, Worcester, Massachusetts 01605,; USA.
 SO    Clin Diagn Lab Immunol. 1994 May;1(3):283-9. Unique Identifier :
       AIDSLINE MED/96050824
 AB    Autologous, virus-transformed lymphoblastoid cell lines were established
       by using peripheral blood lymphocytes from rhesus monkeys that were
       previously immunized with recombinant human immunodeficiency virus type
       1 strain IIIB glycoprotein 160. These autologous cell lines were used to
       present human immunodeficiency virus type 1 viral antigens in a
       processed and cell-associated manner to T lymphocytes. This was
       accomplished by either infecting the cells with recombinant vaccinia
       viruses or pulsing them with synthetic peptides and then subjecting them
       to a mild fixation step with glutaraldehyde. Fixed antigen-presenting
       cells were then used as stimulator cells in vitro to measure
       cell-mediated immune responses. Both the vaccinia virus-infected and
       peptide-pulsed autologous cells stimulated antigen-specific cellular
       proliferative responses. The magnitude of the responses correlated with
       the immunization histories of the animals and other measures of
       immunity, such as antibody titers. Autologous vaccinia virus-infected
       cells were also capable of inducing the in vitro maturation of CD4+ and
       CD8+ precursor cytotoxic T lymphocytes into antigen-specific mature
       cytotoxic T lymphocytes. The use of stimulator cells to present viral
       peptides in a cell-associated manner appeared to be a very sensitive and
       versatile manner in which to measure cell-mediated immune responses with
       peripheral blood lymphocytes from nonhuman primates. It is likely that a
       similar approach will function with peripheral blood lymphocytes from
       humans.
 DE    Amino Acid Sequence  Animal  Antigen Presentation  Antigen-Presenting
       Cells/*IMMUNOLOGY  Cell Transformation, Viral  *Cytotoxicity Tests,
       Immunologic  Epitopes/IMMUNOLOGY  Fixatives/PHARMACOLOGY
       Glutaral/PHARMACOLOGY  HIV Envelope Protein gp120  HIV-1/*IMMUNOLOGY
       Lymphocyte Culture Test, Mixed  *Lymphocyte Transformation/DRUG EFFECTS
       Macaca mulatta  Molecular Sequence Data  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes, Cytotoxic/IMMUNOLOGY  Vaccinia Virus/IMMUNOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

