       Document 0416
 DOCN  M9630416
 TI    Preferential expression of V beta gene families in CD8 memory cells of
       apparently healthy donors.
 DT    9603
 AU    Bonfert V; Cihak J; Losch U; Ziegler-Heitbrock HW; Institute for Animal
       Physiology, University of Munich, Federal; Republic of Germany.
 SO    Cell Immunol. 1995 Dec;166(2):165-71. Unique Identifier : AIDSLINE
       MED/96102264
 AB    Antigen stimulation may lead to expansion or deletion of T-cells
       expressing T-cell receptors that belong to specific V beta gene
       families. Since such stimulation at the same time will lead to
       conversion from naive to memory T-cells, we have asked whether a bias in
       V beta families can be observed when comparing these two populations. We
       have studied the expression of V beta 3, 8, 13.3, 19, and 22 in
       peripheral blood T-cells for 12 apparently healthy male donors. For flow
       cytometry 100,000 CD4+ or CD8+ cells each were analysed in three-colour
       immunofluorescence for percentage of V beta families among CD45R0- naive
       and CD45R0+ memory cell. Greater than twofold excess was found in the
       CD8+CD45R0+ cells in four cases (1 x V beta 13.3, 2 x V beta 19, 1 x V
       beta 22) and a greater than twofold decrease in CD8+CD45R0+ cells in two
       cases (1 x V beta 8, 1 x V beta 22). In contrast, among CD4+ cells no
       such bias was detected. The excess in CD8+CD45R0+ memory cells showed no
       substantial fluctuation over time in that it was found to be stable for
       19 to 70 days. Finally, in vitro conversion of purified CD8+CD45R0-
       cells to CD45R0+ cells by polyclonal stimulation with PHA did not result
       in the excess of V beta usage observed in vivo. These data suggest that
       specific antigen stimulation during past infection or allergy may be
       responsible for the excess of certain V beta gene families. Clinical
       studies looking for disease associations will have to test CD4 and CD8
       naive and memory subsets in order to precisely identify a bias in V beta
       usage and these studies will have to consider the pronounced changes
       observed in healthy controls.
 DE    Adult  Antigens, CD45/GENETICS  CD8-Positive T-Lymphocytes/*IMMUNOLOGY
       Gene Expression Regulation/*IMMUNOLOGY  *Genes, Reiterated  Human
       Immunity, Cellular/GENETICS  Immunologic Memory/*GENETICS  Lymphocyte
       Transformation/GENETICS  Male  Receptors, Antigen, T-Cell,
       alpha-beta/*GENETICS  Support, Non-U.S. Gov't  Time Factors  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

