       Document 0415
 DOCN  M9630415
 TI    HIV-induced syncytia in peripheral blood cell cultures crawl by
       extending giant pseudopods.
 DT    9603
 AU    Shutt DC; Stapleton JT; Kennedy RC; Soll DR; Department of Biological
       Sciences, University of Iowa, Iowa City; 52242, USA.
 SO    Cell Immunol. 1995 Dec;166(2):261-74. Unique Identifier : AIDSLINE
       MED/96102274
 AB    It was previously demonstrated that HIV-induced syncytia of the
       immortalized T cell line SupT1 reorganize their cytoskeleton and form a
       spherical supernuclear complex, thus mimicking the organization,
       polarity, and morphology of a single SupT1 cell. Then, through extension
       of a single, giant pseudopod, these syncytia, which grow to more than
       100 times the volume of a single SupT1 cell, translocate along a
       substratum. To verify that syncytium motility is not peculiar to the
       SupT1 cell line, we have analyzed the cytoskeletal organization and
       motile capabilities of HIV-induced syncytia formed in peripheral blood
       cell cultures containing more than 90% CD4-positive cells. The results
       demonstrate that although peripheral blood T cells differ from SupT1
       cells in size and morphology, they are continuously motile and
       translocate along a substratum in a manner quite similar to that of
       SupT1 cells, and peripheral blood T cell syncytia induced by HIV-1LAI as
       well as two additional clinical isolates translocate by the extension of
       a giant anterior pseudopod in a fashion indistinguishable from that of
       HIV-induced SupT1 syncytia. Together, these results support the
       generalization that HIV-induced T cell syncytia are motile.
 DE    Adult  Cell Fusion  Cell Line  Cell Movement  Cell Nucleus/PATHOLOGY
       Cytoplasm/PATHOLOGY/VIROLOGY  CD4 Lymphocyte Count  CD4-Positive
       T-Lymphocytes/*PATHOLOGY/VIROLOGY  Giant Cells/*PATHOLOGY/VIROLOGY
       Human  HIV Infections/PATHOLOGY  HIV-1/ISOLATION & PURIF/*PATHOGENICITY
       Male  Pseudopodia/*PATHOLOGY/VIROLOGY  Support, U.S. Gov't, Non-P.H.S.
       Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

