       Document 0400
 DOCN  M9630400
 TI    Capillary and slab gel electrophoresis profiling of oligosaccharides.
 DT    9603
 AU    Guttman A; Starr C; Beckman Instruments, Inc, Fullerton, CA 92634, USA.
 SO    Electrophoresis. 1995 Jun;16(6):993-7. Unique Identifier : AIDSLINE
       MED/96118594
 AB    Capillary electrophoresis with laser-induced fluorescent detection, a
       one-dimensional version of the well-established planar analytical method
       of polyacrylamide gel electrophoresis, has been proven to be a powerful
       new microanalytical method for profiling complex carbohydrates. In this
       paper a comparison is presented between the planar high concentration
       polyacrylamide gel electrophoresis method and capillary electrophoresis
       of different carbohydrates with respect to performance and efficiency.
       N-Linked oligosaccharides were released from several glycoproteins,
       including fetuin, human immunodeficiency virus (HIV) envelope
       recombinant glycoprotein (GP-120), alpha 1-acid glycoprotein and
       ribonuclease B, using recombinant peptide-N-glycosidase F (PNGase F).
       Both separation methods involve labeling of the released carbohydrates
       at the reducing end with the fluorescent dye, disodium
       8-amino-1,3,6-naphthalene trisulfonate (ANTS). Fluorophore labeling was
       followed by separation of the labeled oligosaccharides either by high
       concentration polyacrylamide gel electrophoresis or capillary
       electrophoresis.
 DE    Animal  Cattle  Comparative Study  Electrophoresis, Capillary/*METHODS
       Electrophoresis, Polyacrylamide Gel/*METHODS  Glycoproteins/CHEMISTRY
       Human  Oligosaccharides/*ANALYSIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

