       Document 0395
 DOCN  M9630395
 TI    Analysis of a novel defective HTLV-I provirus and detection of a new
       HTLV-I-induced cellular transcript.
 DT    9603
 AU    Kubota S; Furuta RA; Siomi H; Maki M; Hatanaka M; Laboratory of Human
       Tumer Viruses, Kyoto University, Japan.
 SO    FEBS Lett. 1995 Nov 13;375(1-2):31-6. Unique Identifier : AIDSLINE
       GENBANK/X84993
 AB    HTLV-I generally integrates at least one full-length copy in adult
       T-cell leukemia (ATL) cells. A group of patients without full-length
       provirus have a unique conserved truncation of the provirus which
       retains env-pX-3'LTR. Tumor cells of a patient from this group were
       genetically analyzed. Analysis of the 5' and 3' cellular flanking region
       adjacent to the provirus suggest that the defective provirus was
       integrated immediately downstream of a promoter of an unknown cellular
       gene. The activity of the promoter was weak but was responsive to
       Tax-like HTLV-I LTR. The provirus may have utilized it as a substitute
       for the 5'LTR and thus 3'LTR may have become an alternative promoter for
       the cellular gene, which may give similar viral-cellular interactions to
       that of general cases with full-length proviruses. Surprisingly, the 3'
       cellular flanking region which is thought to be controlled originally by
       the promoter is constitutively expressed specifically in an HTLV-I
       producing ATL cell line HUT1O2G, in which the corresponding region is
       not modified by provirus. The detection of this HTLV-I-induced
       transcript provides a probe to find an HTLV-I inducible unknown cellular
       gene that may be related to the pathogenesis of ATL.
 DE    Adult  Aged  Animal  Base Sequence  Cell Line  Cercopithecus aethiops
       Chloramphenicol Acetyltransferase/BIOSYNTHESIS/METABOLISM  Conserved
       Sequence  Defective Viruses/*GENETICS/METABOLISM  Genes, env  Human
       HTLV-I/*GENETICS/METABOLISM  Kidney  Leukemia, T-Cell/GENETICS/*VIROLOGY
       Middle Age  Molecular Sequence Data  Plasmids
       Proviruses/GENETICS/METABOLISM  Repetitive Sequences, Nucleic Acid
       Restriction Mapping  *Transcription, Genetic  Transfection  Tumor Cells,
       Cultured  *Virus Integration  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

