       Document 0383
 DOCN  M9630383
 TI    Effect of exogenous leukotriene B4 (LTB4) on BALB/c mice splenocyte
       production of Th1 and Th2 lymphokines.
 DT    9603
 AU    Arcoleo F; Milano S; D'Agostino P; Cillari E; Institute of General
       Pathology, University of Palermo, Italy.
 SO    Int J Immunopharmacol. 1995 Jun;17(6):457-63. Unique Identifier :
       AIDSLINE MED/96022693
 AB    The effect of exogenous leukotriene B4 (LTB4) on the production of
       cytokines typical of Th1 (interleukin-2 and interferon-gamma) and Th2
       (interleukin-4 and interleukin-10) lymphocytes was studied. Splenocytes
       were stimulated with concanavalin A (ConA) with or without different
       concentrations of LTB4 (3 x 10(-10) to 3 x 10(-7) M) for various times
       in the presence of BW 755C to inhibit the endogenous synthesis of
       eicosanoids. LTB4 was not able to induce cytokine secretion by itself.
       However, LTB4 augmented ConA spleen cell production of interleukin-2
       (IL-2) and interferon-gamma (IFN-gamma) from Th1 cells and interleukin-4
       (IL-4) and interleukin-10 (IL-10) from Th2 cells more than the controls
       treated with ConA alone. The pre-exposition of splenocytes to LTB4 for 3
       h made these cells more sensitive to ConA in terms of IL-2 and IL-10
       production than those treated with LTB4 at the onset of the incubation
       and maintained during the whole culture period. The results suggest that
       LTB4 may participate as a component of the signal transduction process
       for ConA-induced Th1 and Th2 cytokine production in a time-dependent
       manner.
 DE    Animal  Female  Interferon Type II/BIOSYNTHESIS
       Interleukin-10/BIOSYNTHESIS  Interleukin-2/BIOSYNTHESIS
       Interleukin-4/BIOSYNTHESIS  Leukotriene B4/*PHARMACOLOGY
       Lymphokines/*BIOSYNTHESIS/*DRUG EFFECTS  Mice  Mice, Inbred BALB C
       Pregnancy  Spleen/CYTOLOGY/METABOLISM  Support, Non-U.S. Gov't  Th1
       Cells/*DRUG EFFECTS/METABOLISM  Th2 Cells/*DRUG EFFECTS/METABOLISM
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

