       Document 0379
 DOCN  M9630379
 TI    Alteration of spleen lymphocyte populations in rats with arthritis
       induced by muramyl dipeptide analogue or complete adjuvant.
 DT    9603
 AU    Sugawara T; Miyamoto M; Takada S; Nomura M; Kato M; Drug Safety Research
       Center, Daiichi Pharmaceutical Co., Ltd.,; Tokyo, Japan.
 SO    Int J Tissue React. 1995;17(1):5-13. Unique Identifier : AIDSLINE
       MED/96020392
 AB    To examine the involvement of lymphocytes in the development of
       MDP-Lys(L18)-induced arthritis (MIA) in rats and the exacerbation of MIA
       by cyclosporin A (CsA), we analysed the spleen lymphocyte subset using
       monoclonal antibodies and flow cytometry during the development of
       arthritis and compared the results with those found in adjuvant-induced
       arthritis (AIA). Subcutaneous injection of MDP-Lys(L18) 4 mg/kg to male
       Lewis rats for 14 days caused very slight and quite clear increases in
       tarsal joint thickness on days 8 and 15, respectively. This increase was
       significantly enhanced by co-administration of CsA 10 mg/kg on both of
       these days. Adjuvant intracutaneously injected once increased the
       thickness only on day 15, and this was completely inhibited by CsA. The
       populations of CD4+ and CD8+ cells were increased and decreased,
       respectively, increasing the CD4+/CD8+ ratio, from day 8 in MIA. CsA
       enhanced the MDP-Lys(L18)-induced changes in these populations and
       caused additional decreases in the number of CD5+ cells. Only the CD4+
       cell population was increased on day 15 in AIA, and this increase was
       inhibited by CsA. These results suggest that the spleen lymphocyte
       subsets in MIA have a different role from those in AIA, and that the
       contribution of enhancement of the subset changes to the exacerbating
       effect of CsA on MIA.
 DE    Acetylmuramyl-Alanyl-Isoglutamine/ANALOGS & DERIVATIVES  Adjuvants,
       Immunologic  Animal  Antibodies, Monoclonal  Arthritis,
       Adjuvant/*PATHOLOGY  Cyclosporine/*TOXICITY  CD4-CD8 Ratio  Flow
       Cytometry  Lymphocyte Subsets/*DRUG EFFECTS  Male  Rats  Rats, Inbred
       Lew  Spleen/CYTOLOGY/*DRUG EFFECTS  Tarsus, Animal/PATHOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

