       Document 0352
 DOCN  M9630352
 TI    Antiviral cytotoxic T lymphocytes in vaginal mucosa of simian
       immunodeficiency virus-infected rhesus macaques.
 DT    9603
 AU    Lohman BL; Miller CJ; McChesney MB; California Regional Primate Research
       Center, School of Veterinary; Medicine, University of California-Davis
       95616, USA.
 SO    J Immunol. 1995 Dec 15;155(12):5855-60. Unique Identifier : AIDSLINE
       MED/96094483
 AB    The mucosal immune system of the female reproductive tract is of central
       importance for protection against sexually transmitted diseases,
       including HIV; however, this arm of the immune system remains poorly
       understood. Antiviral CTL responses never have been documented in the
       genital tract and the role of CTL in this anatomic site is unknown. In
       this study, CD8+ intraepithelial lymphocytes (IEL) in the vaginas of six
       simian immunodeficiency virus (SIV)-infected female rhesus macaques were
       identified by immunohistochemistry to be CD2+ and TCR beta-chain+. In
       addition, the majority of CD8+ IEL contained TIA-1+ cytoplasmic granules
       that are associated with CTL activity. CD8+ T cells were isolated from
       the vaginal epithelium and submucosa and amplified by limiting dilution
       in the presence of feeder cells. SIV p55gag and/or gp160env-specific
       lysis was detected in cultures of vaginal epithelial but not submucosal
       CD8+ T lymphocytes. Estimated SIV-specific precursor CTL frequencies
       were higher in the vaginal CD8+ IEL population of chronically infected
       monkeys than in the same cells from acutely infected monkeys or a naive
       control monkey. These results provide the first demonstration that
       antiviral CTL are present in the vaginal epithelium, and suggest that a
       vaccine may be able to generate anti-HIV CTL in the genital mucosa.
 DE    Animal  Epithelium/IMMUNOLOGY/VIROLOGY  Female  Gene Products,
       gag/IMMUNOLOGY  Lymphoid Tissue/IMMUNOLOGY  Macaca mulatta  Mucous
       Membrane/IMMUNOLOGY  Receptors, Antigen, T-Cell, alpha-beta/BIOSYNTHESIS
       Simian Acquired Immunodeficiency Syndrome/*IMMUNOLOGY  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, P.H.S.  SAIDS Vaccines/IMMUNOLOGY
       SIV/ISOLATION & PURIF  T-Lymphocytes, Cytotoxic/*IMMUNOLOGY
       Vagina/*IMMUNOLOGY/VIROLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

