       Document 0351
 DOCN  M9630351
 TI    Micro-carrier-test: evaluating disinfectants for HIV.
 DT    9603
 AU    Shimakoshi Y; Second Department of Internal Medicine, Osaka Medical
       College,; Japan.
 SO    Kansenshogaku Zasshi. 1995 Oct;69(10):1151-8. Unique Identifier :
       AIDSLINE MED/96081555
 AB    To determine the effect of disinfectants against viruses in vitro, I
       devised the Micro-Carrier-Test of dry-fixed virus-infected cells. Human
       immunodeficiency virus type 1-infected Molt-4 cells (1 x 10(5) cells in
       5 microliters of 10% fetal bovine serum) were dry-fixed at the bottom of
       each well of a 96-well flat-bottomed microtiter plate for 120 minutes at
       room temperature. Disinfectants were added and allowed to remain for
       designated times and the wells were washed three times with PBS.
       Uninfected Molt-4 cells (1 x 10(4) cells/well) were inoculated and
       cultured for 4 weeks. The culture supernatant was harvested to measure
       reverse transcriptase activity by non-radioisotopic reverse
       transcriptase assay every week. Residual cytotoxicity of the
       disinfectant was determined by cytotoxicity assay. To evaluate the new
       method, the virucidal efficacy of several well-known disinfectants was
       reevaluated. Dose- and time-dependent effects of the disinfectants were
       determined. The minimal effective concentrations after 5 minutes of
       contact were 20% ethanol, 0.01% glutaraldehyde and 0.1% sodium
       hypochlorite. These results are almost the same as those reported
       previously, but there are some discrepancies. The differences between
       the present and previous protocols are discussed. This
       Micro-Carrier-Test promises to be useful in the screening of
       disinfectants.
 DE    Alcohol, Ethyl  Cell Line/MICROBIOLOGY  Disinfectants/*STANDARDS
       Evaluation Studies  Formaldehyde  Glutaral  Human  HIV/*GROWTH &
       DEVELOPMENT  Microbial Sensitivity Tests  Sodium Hypochlorite  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

