       Document 0348
 DOCN  M9630348
 TI    Not only Th2 cells but also Th1 and Th0 cells express CD30 after
       activation.
 DT    9603
 AU    Bengtsson A; Johansson C; Linder MT; Hallden G; van der Ploeg I;
       Scheynius A; Department of Laboratory Medicine, Karolinska Hospital,;
       Stockholm, Sweden.
 SO    J Leukoc Biol. 1995 Dec;58(6):683-9. Unique Identifier : AIDSLINE
       MED/96107361
 AB    To investigate whether the CD30 molecule, expressed only by a minority
       of T and B cells, defines a subtype of T helper cells, Pityrosporum
       orbiculare-specific CD4+ T cell clones were assessed for CD30 protein
       and gene expression. The clones were defined as Th1, Th0, and Th2
       according to their cytokine mRNA profile detected by reverse
       transcription PCR (RT-PCR). The kinetics of CD30 expression after OKT3
       (anti-CD3) stimulation was analyzed by flow cytometry,
       immunocytochemistry, and RT-PCR. OKT3 activation induced a high
       expression of CD30 in cells of both Th1 and Th0 as well as Th2 type
       after 1-3 days. A difference between the clones was noted in that the
       Th2 clones remained highly positive in CD30 expression, whereas
       expression in the other clones started to decline from day 3. These data
       indicate that CD30 is expressed in activated CD4+ T cells of all three
       subtypes, and that the expression is sustained in Th2 cells.
 DE    Antigens, CD30/*ANALYSIS/GENETICS  Base Sequence  Clone Cells
       CD4-Positive T-Lymphocytes/*IMMUNOLOGY  Human  Interleukin-13/GENETICS
       *Lymphocyte Transformation  Molecular Sequence Data  RNA,
       Messenger/ANALYSIS  Support, Non-U.S. Gov't  Th1 Cells/*IMMUNOLOGY  Th2
       Cells/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

