       Document 0328
 DOCN  M9630328
 TI    Is Kaposi's-sarcoma-associated herpesvirus detectable in semen of
       HIV-infected homosexual men?
 DT    9603
 AU    Lin JC; Lin SC; Mar EC; Pellett PE; Stamey FR; Stewart JA; Spira TJ;
       Centers for Disease Control and Prevention, Atlanta, GA 30333,; USA.
 SO    Lancet. 1995 Dec 16;346(8990):1601-2. Unique Identifier : AIDSLINE
       MED/96106230
 AB    We explored a possible route of transmission of
       Kaposi's-sarcoma-associated herpes virus (KSHV) with nested and unnested
       PCR techniques. We looked for KSHV DNA sequences in semen of
       HIV-positive homosexual men and HIV-negative healthy semen donors. With
       unnested primers we found KSHV sequences in 21 of 33 (64%) homosexual
       men and in none of 30 healthy donors. With a nested PCR assay, 30 of 33
       (91%) specimens from the homosexual men and 7 of 30 (23%) specimens from
       healthy donors had detectable KSHV sequences. Over 5 years of follow-up,
       13 of 30 KSHV-positive homosexual men (43%) developed KS compared with
       none of the 3 KSHV-negative homosexual men.
 DE    DNA, Viral/GENETICS  Herpesviridae/GENETICS/*ISOLATION & PURIF
       *Homosexuality, Male  Human  HIV Infections/*COMPLICATIONS/VIROLOGY  HIV
       Seronegativity  Male  Polymerase Chain Reaction  Retrospective Studies
       Sarcoma, Kaposi's/ETIOLOGY/*VIROLOGY  Semen/*VIROLOGY  Sequence
       Analysis, DNA  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

