       Document 0324
 DOCN  M9630324
 TI    Detection of specific human immunodeficiency virus IgM antibodies.
 DT    9603
 AU    Hampl H; Kapprell HP; Sawitzky D; Wilske W; Gurtler L; Abbott GmbH
       Diagnostika, Wiesbaden, Germany.
 SO    Med Microbiol Immunol (Berl). 1995 Aug;184(2):69-71. Unique Identifier :
       AIDSLINE MED/96085966
 AB    This study was done to demonstrate whether the use of the
       antigen-sandwich human immunodeficiency virus (HIV) antibody-screening
       assays (3rd generation assays), which detect all classes of anti-HIV
       immunoglobulins, leads to an earlier detection of HIV IgM compared to
       the 2nd generation HIV antibody-screening assays. We tested sequential
       bleeds of three donors obtained from commercially available
       seroconversion panels. Anti-HIV testing was done before and after
       high-performance liquid chromatography separation of IgG and IgM
       fractions. The positive result of the first bleedings from all three
       panels was linked to the IgM fraction, while at that time the IgG
       fraction was still negative. For subsequent samples drawn 5-9 days
       later, a positive signal was obtained with the IgG fraction in addition
       to a stronger positive signal obtained with the IgM fraction. We
       conclude that an assay capable of simultaneously detecting different
       immunoglobulin classes, including IgM, will help to narrow the window
       period for serological detection of seroconversion to HIV by detecting
       anti-HIV IgM-containing samples earlier than conventional assays using
       only anti-human IgG enzyme conjugates (indirect anti-HIV-screening
       assay, 2nd generation assays).
 DE    Human  HIV Antibodies/*BLOOD  HIV Core Protein p24/BLOOD  IgG/BLOOD
       IgM/*BLOOD  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

