       Document 0266
 DOCN  M9630266
 TI    Induction of IgA and IgG antibodies in vaginal fluid, serum and saliva
       following immunization of genital and gut associated lymphoid tissue.
 DT    9603
 AU    Bergmeier LA; Tao L; Gearing AJ; Adams S; Lehner T; Division of
       Immunology, UMDS of Guy's and St. Thomas's Hospital,; London.
 SO    Adv Exp Med Biol. 1995;371B:1567-73. Unique Identifier : AIDSLINE
       MED/96001796
 AB    Vaginal immunization with the Simian immunodeficiency virus (SIV) was
       investigated in macaques in order to study genital mucosal antibodies. A
       combined route of genital- and gut-associated lymphoid tissues was used
       to stimulate IgA and IgG antibodies in vaginal fluid, serum and saliva.
       Macaques were immunized with a recombinant, particulate SIV antigen (SIV
       gag P27), covalently linked to the mucosal adjuvant cholera toxin B
       subunit (CTB). The animals were immunized sequentially as follows:
       vaginal (x2) followed by oral (x3), or the reverse sequence of
       immunization. The results show that both vaginal followed by oral
       immunization or the reverse sequence induces specific p27 IgA and IgG
       antibodies in the vaginal fluid and serum. IgA antibodies were also
       detected in saliva. Vaginal IgA antibodies have the secretory component
       and J chain suggesting that they are of secretory origin.
 DE    Adjuvants, Immunologic/ADMINISTRATION & DOSAGE  Administration,
       Intravaginal  Administration, Oral  Animal  Antigens,
       Viral/ADMINISTRATION & DOSAGE  Cholera Toxin/ADMINISTRATION &
       DOSAGE/IMMUNOLOGY  Female  Gene Products, gag/ADMINISTRATION &
       DOSAGE/IMMUNOLOGY  Human  HIV Infections/IMMUNOLOGY/PREVENTION &
       CONTROL/TRANSMISSION  IgA/*BIOSYNTHESIS/*BLOOD  IgA,
       Secretory/*BIOSYNTHESIS  IgG/*BIOSYNTHESIS/*BLOOD  Immunity, Mucosal
       Immunization  Intestinal Mucosa/IMMUNOLOGY  Lymphoid Tissue/IMMUNOLOGY
       Macaca  Saliva/*IMMUNOLOGY  SAIDS Vaccines/ADMINISTRATION & DOSAGE
       SIV/IMMUNOLOGY  Vagina/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

