       Document 0253
 DOCN  M9630253
 TI    Costimulation of CD3/TcR complex with either integrin or nonintegrin
       ligands protects CD4+ allergen-specific T-cell clones from programmed
       cell death.
 DT    9603
 AU    Agea E; Bistoni O; Bini P; Migliorati G; Nicoletti I; Bassotti G;
       Riccardi C; Bertotto A; Spinozzi F; Department of Internal Medicine,
       University of Perugia, Italy.
 SO    Allergy. 1995 Aug;50(8):677-82. Unique Identifier : AIDSLINE
       MED/96098155
 AB    An optimal stimulation of CD4+ cells in an immune response requires not
       only signals transduced via the TcR/CD3 complex, but also costimulatory
       signals delivered as a consequence of interactions between T-cell
       surface-associated costimulatory receptors and their counterparts on
       antigen-presenting cells (APC). The intercellular adhesion molecule-1
       (ICAM-1, CD54) efficiently costimulates proliferation of resting, but
       not antigen-specific, T cells. In contrast, CD28 and CD2 support
       interleukin (IL)-2 synthesis and proliferation of antigen-specific T
       cells more efficiently than those of resting T cells. The molecular
       basis for this differential costimulation of T cells is poorly
       understood. Cypress-specific T-cell clones (TCC) were generated from
       four allergic subjects during in vivo seasonal exposure to the allergen.
       Purified cypress extract was produced directly from fresh collected
       pollen and incubated with the patients' mononuclear cells. Repeated
       allergen stimulation was performed in T-cell cultures supplemented with
       purified extract and autologous APC. The limiting-dilution technique was
       then adopted to generate allergen-specific TCC, which were also
       characterized by their cytokine secretion pattern as Th0 (IL-4 plus
       interferon-gamma) or Th2 (IL-4). Costimulation-induced proliferation or
       apoptosis was measured by propidium iodide cytofluorometric assay. By
       cross-linking cypress-specific CD4+ and CD8+ T-cell clones with either
       anti-CD3 or anti-CD2, anti-CD28, and anti-CD54 monoclonal antibodies, we
       demonstrated that CD4+ clones (with Th0- or Th2-type cytokine production
       pattern) undergo programmed cell death only after anti-CD3 stimulation,
       whereas costimulation with either anti-CD54 or anti-CD28 protects target
       cells from apoptosis.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Allergens/*IMMUNOLOGY  Antigen-Presenting Cells/IMMUNOLOGY  Antigens,
       CD2/IMMUNOLOGY  Antigens, CD28/IMMUNOLOGY  Antigens, CD3/*IMMUNOLOGY
       Apoptosis/*PHYSIOLOGY  Cell Division  Cells, Cultured  Clone Cells
       CD4-Positive T-Lymphocytes/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Hay Fever/IMMUNOLOGY  Human
       Integrins/*IMMUNOLOGY  Intercellular Adhesion Molecule-1/IMMUNOLOGY
       Interleukin-1/METABOLISM  Pollen/IMMUNOLOGY  Receptors, Antigen,
       T-Cell/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

