       Document 0243
 DOCN  M9630243
 TI    Human immunodeficiency virus nucleocapsid protein stimulates strand
       transfer from internal regions of heteropolymeric RNA templates.
 DT    9603
 AU    DeStefano JJ; Department of Microbiology, University of Maryland,
       College Park,; USA.
 SO    Arch Virol. 1995;140(10):1775-89. Unique Identifier : AIDSLINE
       MED/96105336
 AB    We have examined the influence of HIV nucleocapsid protein (NCp) on
       strand transfer from internal regions of a heteropolymeric RNA template.
       The system consisted of a DNA-primed 225 nucleotide RNA donor template,
       on which reverse transcriptase initiated primer extension, and a 189
       nucleotide RNA acceptor template, to which extended primers could
       transfer. The last 133 nucleotides on the 3' end of the acceptor were
       homologous to an internal region on the donor, limiting homologous
       strand transfer to this region. Primers extended to the end of the donor
       were 212 nucleotides while those transferred to, and extended on the
       acceptor were 259 nucleotides in length. The rate of strand transfer and
       the level of transfer products increased several-fold when nucleocapsid
       was included in the reactions. The increase was due, at least in part,
       to the transfer to, and extension on the acceptor, of incompletely
       elongated primer-extension products that were chased into transfer
       products in the presence of nucleocapsid. Nucleocapsid did not directly
       influence reverse transcriptase elongation as the enzyme processivity
       (number of nucleotides incorporated before the enzyme dissociates from
       the primer-template) was approximately the same in the presence and
       absence of nucleocapsid.
 DE    Capsid/*METABOLISM  Cell-Free System  DNA, Viral/METABOLISM  Gene
       Products, gag/*METABOLISM  HIV-1/*GENETICS  Recombinant Proteins
       RNA-Directed DNA Polymerase/METABOLISM  RNA, Viral/*METABOLISM  Support,
       U.S. Gov't, P.H.S.  Templates  *Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

