       Document 0208
 DOCN  M9630208
 TI    [Activation of cell death in lymphocytes from individuals infected with
       HIV]
 DT    9603
 AU    Quiros E; Lopez-Canabare MJ; Quiros A; Piedrola G; Maroto MC;
       Departamento de Microbiologia, Facultad de Medicina, Granada.
 SO    Enferm Infecc Microbiol Clin. 1995 Nov;13(9):522-6. Unique Identifier :
       AIDSLINE MED/96122978
 AB    BACKGROUND: In HIV infection, T cells depletion cannot only be explained
       by direct viral infection. Programmed cell death (PCD), also know as
       apoptosis or activation-induced cell death, may be responsible for the
       deletion of reactive T cells that contributes to HIV induced
       immunodeficiency. We studied the response of lymphocytes in vitro to
       different polyclonal and oligoclonal (superantigen) activators, and
       tested whether the stimulation of T cells from individual with HIV
       infection lead to cell death. MATERIAL AND METHODS: Peripheral blood
       mononuclear cells, total T lymphocytes, TCD4+ and TCD8+ cells from 10
       positive patients, 2 patients with AIDS and 10 negative individual for
       the virus were cultured in RPMI medium and subjected to stimulation with
       PHA, PWM and SEB (staphylococcus B enterotoxin). Trypan blue was used to
       distinguish viable from dead cells. RESULTS: TCD4+ cells from
       HIV-infected patients die after SEB or PWM activation (almost 40% from
       asymptomatic and 80% from AIDS), and do not die TCD8+ from the same
       individuals (around 10-15%), or T cells from seronegative controls.
       CONCLUSIONS: Lymphocytes from patients with HIV-infection showed as
       abnormal proliferative response to self-MHC class II restricted recall
       antigens and PWM whereas the response to PHA was conserved. Probably,
       apoptosis is triggered by an aberrant form of T cell activation in the
       mature TCD4+ cell population in HIV-infected individuals.
 DE    Acquired Immunodeficiency Syndrome/IMMUNOLOGY  *Apoptosis  CD4-Positive
       T-Lymphocytes/*PHYSIOLOGY  CD8-Positive T-Lymphocytes/PHYSIOLOGY
       English Abstract  Human  HIV Infections/*IMMUNOLOGY  Immunity, Cellular
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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