       Document 0123
 DOCN  M9630123
 TI    Mother-to-child transmission of human T-lymphotropic virus type II.
 DT    9603
 AU    Van Dyke RB; Heneine W; Perrin ME; Rudolph D; Starszak E; Woods T;
       Switzer WM; Kaplan JE; Department of Pediatrics, Tulane University
       School of Medicine,; New Orleans, Louisiana.
 SO    J Pediatr. 1995 Dec;127(6):924-8. Unique Identifier : AIDSLINE
       MED/96101712
 AB    OBJECTIVE: To determine the frequency of mother-to-child transmission of
       human T-lymphotropic virus type II (HTLV-II) and to explore its
       association with breast-feeding. DESIGN: Prospective study of children
       born to a cohort of HTLV-II-infected pregnant women and a
       cross-sectional study of older siblings of these children. METHODS:
       Maternal sera were screened with an HTLV-I enzyme immunoassay that
       detects antibody to both HTLV-I and HTLV-II. Confirmatory serologic
       testing and viral typing were performed by Western blot,
       radioimmunoprecipitation assay, enzyme immunoassay with HTLV
       type-specific proteins, and polymerase chain reaction (PCR) analysis of
       DNA from peripheral blood mononuclear cells. The presence of HTLV was
       evaluated in children by serial serologic and PCR testing. Molecular
       analysis of PCR products from infected mother-child pairs was performed
       by means of restriction fragment length polymorphism of HTLV-II
       long-terminal repeated sequences. RESULTS: Twenty-nine HTLV-II-infected
       women were identified, and these 29 women had 30 pregnancies during the
       study. Of 28 live infants born to infected women, 19 were examined and
       none was infected with HTLV-II. Sixteen older children less than 10
       years of age who were born previously to the infected women were also
       examined; two were infected with HTLV-II. One infected child was breast
       fed for 2 months and the second was not breast fed. The viral patterns
       of restriction fragment length polymorphism in the two infected children
       were distinct, but the viral pattern in each child was identical to that
       of her mother's virus, suggesting mother-to-child transmission. Overall,
       among examined children, 1 of 7 breast-fed children (14%; 95% confidence
       interval: 0, 40) and 1 of 28 children who were not breast fed (3.6%; 95%
       confidence interval: 0, 10) were infected with HTLV-II. CONCLUSION:
       Mother-to-child transmission of HTLV-II occurs both with and without
       breast-feeding and at rates similar to those of HTLV-I. We believe that
       this is the first demonstration of mother-to-child transmission of
       HTLV-II in the absence of breast-feeding.
 DE    Blotting, Western  Breast Feeding  Child  Child, Preschool  Cohort
       Studies  Cross-Sectional Studies  Female  Human  HIV-1/ISOLATION & PURIF
       HTLV-I/ISOLATION & PURIF  HTLV-II/*ISOLATION & PURIF  Immunoenzyme
       Techniques  Infant  *Maternal-Fetal Exchange  Polymerase Chain Reaction
       Polymorphism, Restriction Fragment Length  Pregnancy  Prospective
       Studies  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

