       Document 0120
 DOCN  M9630120
 TI    Evidence that the murine AIDS defective virus does not encode a
       superantigen.
 DT    9603
 AU    Doyon L; Simard C; Sekaly RP; Jolicoeur P; Laboratoire d'Immunologie,
       Institut de Recherches Cliniques de; Montreal, Canada.
 SO    J Virol. 1996 Jan;70(1):1-9. Unique Identifier : AIDSLINE MED/96099406
 AB    The T-cell receptor repertoire was analyzed in C57BL/6 mice upon
       infection with helper-free stocks of the pathogenic murine AIDS (MAIDS)
       defective virus in order to demonstrate if, as previously reported, this
       virus encodes a superantigen. A polyclonal T-cell stimulation involving
       T cells expressing multiple V beta subsets occurred within the first
       week of infection, while late in the disease we could note only a 50%
       deletion of V beta 5 CD8+ cells. Transfection of the MAIDS virus genomic
       DNA into fibroblasts and B cells expressing major histocompatibility
       complex class II molecules failed to show any stimulation of cells
       expressing the specific V beta (V beta 5) previously reported to respond
       to MAIDS virus-infected cells. In addition, mice lacking V beta 5 cells
       did not show any significant decrease in susceptibility to the disease
       compared with mice expressing V beta 5 and bred on the same genetic
       background. Our in vivo and in vitro results fail to demonstrate a role
       for a superantigen encoded by the MAIDS defective viral genome in the
       pathogenesis of MAIDS.
 DE    Animal  B-Lymphocytes/IMMUNOLOGY/VIROLOGY  Cell Line  CD8-Positive
       T-Lymphocytes/METABOLISM  Defective Viruses/*IMMUNOLOGY/ISOLATION &
       PURIF  Disease Susceptibility/IMMUNOLOGY  Fibroblasts/CYTOLOGY  Helper
       Viruses  Histocompatibility Antigens Class II  Human  Leukemia Viruses,
       Murine/*IMMUNOLOGY/ISOLATION & PURIF  Mice  Mice, Inbred C57BL  Mice,
       Transgenic  Murine Acquired Immunodeficiency
       Syndrome/*IMMUNOLOGY/VIROLOGY  Receptors, Antigen, T-Cell/IMMUNOLOGY
       Receptors, Antigen, T-Cell, alpha-beta/IMMUNOLOGY
       Superantigens/BIOSYNTHESIS/*IMMUNOLOGY  Support, Non-U.S. Gov't
       T-Lymphocytes/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

