       Document 0118
 DOCN  M9630118
 TI    RNA aptamers selected to bind human immunodeficiency virus type 1 Rev in
       vitro are Rev responsive in vivo.
 DT    9603
 AU    Symensma TL; Giver L; Zapp M; Takle GB; Ellington AD; Department of
       Chemistry, Indiana University, Bloomington 47405,; USA.
 SO    J Virol. 1996 Jan;70(1):179-87. Unique Identifier : AIDSLINE
       MED/96099429
 AB    RNA aptamers (binding sequences) that can interact tightly and
       specifically with the human immunodeficiency virus type 1 Rev protein
       have previously been selected from random sequence pools. Although the
       selected sequences compete with the wild-type Rev-binding element (RBE)
       in vitro, it was not known whether they would be able to functionally
       replace the RBE in vivo. Two aptamers that were different from the
       wild-type RBE in terms of both primary sequence and secondary structure
       were inserted into the full-length Rev-responsive element (RRE) in place
       of the RBE. The hybrid RREs were assayed for their ability to mediate
       Rev function in vivo using a reporter system. The aptamers were found to
       be functionally equivalent to the wild-type element when the assay
       system was saturated with Rev and better than the wild-type element when
       Rev was limiting. These results demonstrate that the affinity of the
       primary Rev-binding element rather than its particular sequence may be
       most responsible for conferring Rev responsiveness on viral mRNAs.
       Moreover, the fact that increased binding ability can lead to increased
       Rev responsiveness suggests that cellular factors do not directly
       influence the Rev:RBE interaction. Finally, since sequences distinct
       from the RBE are found to be Rev responsive, it may be possible for the
       RBE to readily mutate in response to drugs or gene therapy reagents that
       target the Rev:RBE interaction.
 DE    Animal  Base Sequence  Binding Sites  Cell Line  Cercopithecus aethiops
       DNA, Viral  Gene Products, rev/*GENETICS/METABOLISM  *Genes, rev  Human
       HIV-1/*GENETICS  Molecular Sequence Data  Nucleic Acid Conformation
       RNA, Viral/*METABOLISM  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       Non-P.H.S.  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

