       Document 0108
 DOCN  M9630108
 TI    Inter- and intraclade neutralization of human immunodeficiency virus
       type 1: genetic clades do not correspond to neutralization serotypes but
       partially correspond to gp120 antigenic serotypes.
 DT    9603
 AU    Moore JP; Cao Y; Leu J; Qin L; Korber B; Ho DD; Aaron Diamond AIDS
       Research Center, New York University School of; Medicine, New York
       10016, USA.
 SO    J Virol. 1996 Jan;70(1):427-44. Unique Identifier : AIDSLINE
       MED/96099458
 AB    We have studied genetic variation among clades A through E of human
       immunodeficiency virus type 1 (HIV-1) at the levels of antibody binding
       to gp120 molecules and virus neutralization. We are unable to identify
       neutralization serotypes that correspond to the genetic clades. Instead,
       we observe that inter- and intraclade neutralization of primary isolates
       by HIV-1-positive sera is generally weak and sporadic; some sera show a
       reasonable degree of neutralization breadth and potency whereas others
       are relatively sensitive to neutralization, but no consistent pattern
       was found. However, a few sera were able to neutralize across clades
       with significant potency, an observation which may have implications for
       the feasibility of a broadly effective HIV-1 vaccine involving humoral
       immunity. Serological assays measuring anti-gp120 antibody binding also
       failed to identify serotypes that correspond precisely to the genetic
       clades, but some indications of clade-specific binding were observed,
       notably with sera from clades B and E. A representative protein for each
       clade (A through E) was selected on the basis of its specificity,
       defined as high seroreactivity with sera from individuals infected with
       virus of that clade and lower reactivity with sera from individuals
       infected with viruses from other clades. The seroreactivity patterns
       against these five proteins could be used to predict the genotype of the
       infecting virus with moderate success.
 DE    Amino Acid Sequence  Antibodies, Viral/IMMUNOLOGY  Human  HIV Envelope
       Protein gp120/*IMMUNOLOGY  HIV Seropositivity/IMMUNOLOGY/VIROLOGY
       HIV-1/CLASSIFICATION/GENETICS/*IMMUNOLOGY  Molecular Sequence Data
       Neutralization Tests  Serotyping  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.  Variation (Genetics)
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

