       Document 0105
 DOCN  M9630105
 TI    Primary characterization of a herpesvirus agent associated with Kaposi's
       sarcoma.
 DT    9603
 AU    Moore PS; Gao SJ; Dominguez G; Cesarman E; Lungu O; Knowles DM; Garber
       R; Pellett PE; McGeoch DJ; Chang Y; Division of Epidemiology, Columbia
       University, New York, New York; 10032, USA.
 SO    J Virol. 1996 Jan;70(1):549-58. Unique Identifier : AIDSLINE
       GENBANK/U40377
 AB    Detection of novel DNA sequences in Kaposi's sarcoma (KS) and
       AIDS-related body cavity-based, non-Hodgkin's lymphomas suggests that
       these neoplasms are caused by a previously unidentified human
       herpesvirus. We have characterized this agent using a continuously
       infected B-lymphocyte cell line derived from an AIDS-related lymphoma
       and a genomic library made from a KS lesion. In this cell line, the
       agent has a large episomal genome with an electrophoretic mobility
       similar to that of 270-kb linear DNA markers during clamped homogeneous
       electric field gel electrophoresis. A 20.7-kb region of the genome has
       been completely sequenced, and within this region, 17 partial and
       complete open reading frames are present; all except one have sequence
       and positional homology to known gammaherpesvirus genes, including the
       major capsid protein and thymidine kinase genes. Phylogenetic analyses
       using both single genes and combined gene sets demonstrated that the
       agent is a gamma-2 herpesvirus (genus Rhadinovirus) and is the first
       member of this genus known to infect humans. Evidence for transient
       viral transmission from infected to uninfected cells is presented, but
       replication-competent virions have not been identified in infected cell
       lines. Sera from patients with KS have specific antibodies directed
       against antigens of infected cell lines, and these antibodies are
       generally absent in sera from patients with AIDS without KS. These
       studies define the agent as a new human herpesvirus provisionally
       assigned the descriptive name KS-associated herpesvirus; its formal
       designation is likely to be human herpesvirus 8.
 DE    Amino Acid Sequence  Animal  Base Sequence  Cell Line  Cohort Studies
       DNA, Viral  Gammaherpesvirinae/CLASSIFICATION/GENETICS/*ISOLATION &
       PURIF  Genome, Viral  Human  HIV Antibodies/BLOOD  Lymphoma,
       AIDS-Related/VIROLOGY  Molecular Sequence Data  Phylogeny  Sarcoma,
       Kaposi's/IMMUNOLOGY/*VIROLOGY  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

