       Document 0104
 DOCN  M9630104
 TI    Relationship between tumor necrosis factor alpha and feline
       immunodeficiency virus expressions.
 DT    9603
 AU    Kraus LA; Bradley WG; Engelman RW; Brown KM; Good RA; Day NK; Department
       of Medical Microbiology and Immunology, University of; South Florida,
       Tampa 33612, USA.
 SO    J Virol. 1996 Jan;70(1):566-9. Unique Identifier : AIDSLINE MED/96099471
 AB    The presence of feline immunodeficiency virus (FIV) proviral DNA,
       expression of FIV p26 core protein, and production of tumor necrosis
       factor alpha (TNF-alpha) were assessed in sequential biopsies of spleen
       and lymph node sections, of mononuclear cells of the peripheral blood,
       and of the serum of specific-pathogen-free cats during the acute phase
       of FIV infection. A temporal relationship between TNF-alpha production
       and FIV p26 expression was noted. Two months following FIV infection,
       and preceding the detection of FIV viremia, levels of TNF-alpha in serum
       increased significantly (P = 0.04), and they remained elevated during
       FIV viremia in the third month postinfection. Immunoprecipitates
       representing expression of TNF-alpha and of FIV p26 were localized in
       common foci of lymph nodes of FIV-infected cats during this period of
       active viremia. With the advent of anti-FIV antibodies, circulating
       levels of TNF-alpha and p26 antigen and expression of TNF-alpha and p26
       in the lymph nodes decreased during the fifth month postinfection, and
       p26 production became undetectable. With clearance of viremia, burden of
       proviral DNA in peripheral blood mononuclear cells became reduced (P =
       0.041), with provirus remaining integrated principally within lymph
       nodes (P = 0.046). During aviremia, p26 expression was undetectable in
       any tissue but remained inducible in vitro. During acute FIV infection,
       TNF-alpha production and p26 expression are intimately linked.
 DE    Animal  Antibodies, Viral/BLOOD  Base Sequence  Cats  DNA, Viral  Feline
       Acquired Immunodeficiency Syndrome/*IMMUNOLOGY  Immunodeficiency Virus,
       Feline/*IMMUNOLOGY  Lymph Nodes/CYTOLOGY/IMMUNOLOGY  Molecular Sequence
       Data  Proviruses/IMMUNOLOGY  Spleen/CYTOLOGY/IMMUNOLOGY  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Tumor Necrosis
       Factor/*BIOSYNTHESIS  Viral Core Proteins/BIOSYNTHESIS/IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

