       Document 0099
 DOCN  M9630099
 TI    Specific binding of human immunodeficiency virus type 1 (HIV-1)
       Gag-derived proteins to a 5' HIV-1 genomic RNA sequence.
 DT    9603
 AU    Geigenmuller U; Linial ML; Division of Basic Sciences, Fred Hutchinson
       Cancer Research; Center, Seattle, Washington 98104, USA.
 SO    J Virol. 1996 Jan;70(1):667-71. Unique Identifier : AIDSLINE
       MED/96099490
 AB    We developed an in vitro binding assay to study the specific interaction
       between human immunodeficiency virus type 1 (HIV-1) RNA and the Gag
       polyprotein. Binding of the in vitro-expressed protein to in
       vitro-transcribed RNA was determined by altered migration of the protein
       in polyacrylamide gels. We found that a Gag precursor lacking the matrix
       domain bound specifically to HIV-1 RNA, while deletion of both matrix
       and capsid domains diminished the specificity of binding. Among several
       regions of HIV-1 RNA tested, strongest binding was seen with the 5'-most
       261 nucleotides, while antisense RNA from the same region did not bind.
 DE    Animal  Gene Deletion  Gene Products, gag/GENETICS/*METABOLISM  Genome,
       Viral  Human  HIV-1/GENETICS/*METABOLISM  Protein Binding  RNA,
       Viral/*METABOLISM  Structure-Activity Relationship  Support, U.S. Gov't,
       P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

